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. 2021 Feb 10:29:e00598.
doi: 10.1016/j.btre.2021.e00598. eCollection 2021 Mar.

Study of bacterial interactions in reconstituted hydrocarbon-degrading bacterial consortia from a local collection, for the bioremediation of weathered oily-soils

Shaikha Y Alsayegh  1 , Mohammad A Al-Ghouti  1 , Nabil Zouari  1
Affiliations

Study of bacterial interactions in reconstituted hydrocarbon-degrading bacterial consortia from a local collection, for the bioremediation of weathered oily-soils

Shaikha Y Alsayegh et al. Biotechnol Rep (Amst). .

Abstract

To enhance the process of bacterial remediation of weathered hydrocarbons, the area of Dukhan, Qatar, was considered as a model for weathering processes. Self-purification by indigenous hydrocarbon-degrading bacteria showed low performance. Biostimulation/seeding using one or another of the indigenous bacteria improved the performance. Symbiosis between three strains dominating the soil; Bacillus sorensis D11, Bacillus cereus D12, and Pseudomonas stutzeri D13, was highly performant for removal of total petroleum hydrocarbons in the weathered soil. D11, the most sensitive, showed the highest performance when mixed with D12 or D13. D12, less performant than D11, was more active on diesel range organics (DRO: C10-C28), similar to D11. D13 showed a metabolic behavior close to commensal and co-metabolic ones. It was more active on hydrocarbons above C29. Combination of the three strains conducted to the removal of at least 80% of C10-C35 organics in the extract at concentrations of 31.1 mg/g TPH-DRO.

Keywords: Bacillus cereus; Bacillus sorensis; Bioremediation; Weathered hydrocarbons; pseudomonas stutzeri; symbiosis.

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Conflict of interest statement

The authors report no declarations of interest.

Figures

Fig. 1
Fig. 1
Distinctive colony characteristics of the strains D11 Bacillus sorensis, D12 Bacillus cereus, and D13 Pseudomonas stutzeri on solid LB medium.
Fig. 2
Fig. 2
Evaluation of growth in 0.311 mg TPH-DRO corresponding to 198 mg/mL TPH-ORO. Cells counts are presented as 107 cfu/mL. A: Cultures inoculated with separate bacterial strains. B: cultures inoculated with bacterial mixtures: D11 in D11/D12; D11* in D11/D13; D11** in D11+D12+D13; D12 in D12/D11; D12* in D12/D13; D12** in D12/D11/D13; D13 in D13/D11 and D13* in D13 + D12.
Fig. 3
Fig. 3
Evaluation of growth in 1.55 mg/mL TPH-DRO corresponding to 9.9 mg/mL TPH-ORO. Cells counts are presented as 107cfu/mL. A: Cultures inoculated with separate bacterial strains. B: cultures inoculated with bacterial mixtures: D11 in D11/D12; D11* in D11/D13; D11** in D11+D12+D13; D12 inD12/D11; D12* in D12/D13; D12** in D12/D11/D13; D13 in D13/D11 and D13* in D13 + D12.
Fig. 4
Fig. 4
Evaluation of growth in 3.11 mg/mL TPH-DRO corresponding to 19.8 mg/mL TPH-ORO. Cells counts are presented as 107cfu/mL. A: Cultures inoculated with separate bacterial strains. B: cultures inoculated with bacterial mixtures: D11 in D11/D12; D11* in D11/D13; D11** in D11+D12+D13; D12 inD12/D11; D12* in D12/D13; D12** in D12/D11/D13; D13 in D13/D11 and D13* in D13 + D12.
Fig. 5
Fig. 5
Evaluation of growth in 31.1 mg/mL TPH-DRO corresponding to 198 mg/mL TPH-ORO. Cells counts are presented as 107 cfu/mL. A: Cultures inoculated with separate bacterial strains. B: cultures inoculated with bacterial mixtures: D11 in D11/D12; D11* in D11/D13; D11** in D11+D12+D13; D12 inD12/D11; D12* in D12/D13; D12** in D12/D11/D13; D13 in D13/D11 and D13* in D13 + D12.
Fig. 6
Fig. 6
FTIR analyses performed in the cultures performed with : A1: D11 in 1.55 mg TPH-DRO corresponding to 9.9 mg/mL TPH-ORO. A2: D11 in 31.1 mg TPH-DRO corresponding to 198 mg/mL TPH-ORO. B1: D12 in D11 in 1.55 mg TPH-DRO corresponding to 9.9 mg/mL TPH-ORO. B2: D12 in 31.1 mg TPH-DRO corresponding to 198 mg/mL TPH-ORO. C1: D13 in D11 in 1.55 mg TPH-DRO corresponding to 9.9 mg/mL TPH-ORO. C2: D13 in 31.1 mg TPH-DRO corresponding to 198 mg/mL TPH-ORO.. (formula image): Week 2, (formula image): Week 4, (formula image): Week 6 and (formula image): Week 14.
Fig. 7
Fig. 7
FTIR analyses performed in the cultures performed with: A1: D11/D12 in 1.55 mg TPH-DRO corresponding to 9.9 mg/mL TPH-ORO. A2: D11/D12 in 31.1 mg TPH-DRO corresponding to 198 mg/mL TPH-ORO. B1: D11/D13 in 1.55 mg TPH-DRO corresponding to 9.9 mg/mL TPH-ORO. B2: D11/D13 in 31.1 mg TPH-DRO corresponding to 198 mg/mL TPH-ORO. C1: D12/D13 in 1.55 mg TPH-DRO corresponding to 9.9 mg/mL TPH-ORO. C2: D12/D13 in 31.1 mg TPH-DRO corresponding to 198 mg/mL TPH-ORO.. (formula image): Week 2, (formula image): Week 4, (formula image): Week 6 and (formula image): Week 14.
Fig. 8
Fig. 8
FTIR analyses performed in the cultures performed with : A : D11/D12/D3 in 0.311 mg TPH-DRO corresponding to 198 mg/mL TPH-ORO. B: D11/D12/D3 in 1.55 mg TPH-DRO corresponding to 9.9 mg/mL TPH-ORO. (formula image): Week 2, (formula image): Week 4, (formula image): Week 6 and (formula image): Week 14.
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