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. 1988 Apr;24(4):289-93.
doi: 10.1007/BF02628829.

A cloned rat thymic epithelial cell line established from serum-free selective culture

A Piltch  1 , P Naylor, J Hayashi
Affiliations

A cloned rat thymic epithelial cell line established from serum-free selective culture

A Piltch et al. In Vitro Cell Dev Biol. 1988 Apr.

Abstract

A serum-free system has been developed for selective growth and long-term culture of rat thymic epithelial cells. The growth media is a modification of McKeehan's WAJC 404, plus insulin, cholera toxin, dexamethasone, and epidermal growth factor. Cultures have been continuously passaged and maintained for over 6 mo., and a cloned cell line, TEA3A1, has been established. These cells are epithelial, judging by morphology and ultrastructure, and are positive for A2B5 and thymosin alpha 1 markers for thymic endocrine cells.

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References

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