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Review
. 2021 Feb 28;22(5):2417.
doi: 10.3390/ijms22052417.

Capsid and Genome Modification Strategies to Reduce the Immunogenicity of Adenoviral Vectors

Florian Kreppel  1 , Claudia Hagedorn  1
Affiliations
Review

Capsid and Genome Modification Strategies to Reduce the Immunogenicity of Adenoviral Vectors

Florian Kreppel et al. Int J Mol Sci. .

Abstract

Adenovirus-based gene transfer vectors are the most frequently used vector type in gene therapy clinical trials to date, and they play an important role as genetic vaccine candidates during the ongoing SARS-CoV-2 pandemic. Immediately upon delivery, adenovirus-based vectors exhibit multiple complex vector-host interactions and induce innate and adaptive immune responses. This can severely limit their safety and efficacy, particularly after delivery through the blood stream. In this review article we summarize two strategies to modulate Ad vector-induced immune responses: extensive genomic and chemical capsid modifications. Both strategies have shown beneficial effects in a number of preclinical studies while potential synergistic effects warrant further investigations.

Keywords: HPMA; PEGylation; adenovirus; cloaking; immune response; shielding; stealthing; vector.

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Conflict of interest statement

The authors declare no conflict of interest.

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