Synergistic effect of Spirulina platensis and selenium nanoparticles on growth performance, serum metabolites, immune responses, and antioxidant capacity of heat-stressed broiler chickens

Abstract

This study examined the effects of dietary Spirulina platensis (SP) at levels of 0, 5, and 10 g.kg^-1 and selenium nanoparticles (SeNPs) at 0, 0.1, and 0.2 mg.kg^-1, individually and in combination, on heat-stressed broiler chickens for 5 weeks. Four hundred fifty one-day-old Ross-308 chicks were allocated to 9 dietary groups with 5 replicates (10 chicks each). The control diet was consisted of corn-soybean-based basal diet. The obtained results displayed a significant increase in final body weight (p = 0.005) and weight gain during the periods from 22 to 35 days (p = 0.002) and 1 to 35 days (p = 0.005) in birds fed supplemented diets compared to those fed control diet, with the highest being in birds fed with both 10 g SP and 0.1 mg SeNPs. Feed conversion ratio was also improved in birds fed supplemented compared to control group. Dietary supplements significantly improved carcass dressing (p < 0.001), carcass yield (p = 0.001) percentages, and blood lipid profile. Blood triiodothyronine was higher (p = 0.005) with all treated diets except that contain 5 g SP compared to the control, with the highest being in birds fed diet contains 5 g SP + 0.2 mg SeNPs. Immunoglobulin subclasses IgG, IgM, and IgA were higher in birds fed supplemented diets compared to the control group. Antibody titers to Newcastle disease, avian influenza, and infectious bursal disease were numerically increased with dietary supplementation compared to the control group. Dietary treatments increased (p < 0.001) glutathione peroxidase and superoxide dismutase (SOD) levels, except diet contains 5 g SP for SOD level and decreased (p < 0.001) malondialdehyde level. It is concluded that dietary inclusion of SP and SeNPs, particularly their combination at levels 5 g SP plus 0.2 mg SeNPs kg^-1 and 10 g SP plus 0.1 mg SeNPs kg^-1, improved growth performance, carcass yield, immunity, and antioxidant capacity of heat-stressed broilers.

Keywords: Antioxidant; Broilers; Growth; Heat stress; Selenium nanoparticles; Spirulina.