Two-wavelength cytophotometry: the choice of the wavelengths from a practical point of view
- PMID: 336786
- DOI: 10.1177/25.12.336786
Two-wavelength cytophotometry: the choice of the wavelengths from a practical point of view
Abstract
Methods in use for the choice of the two-wavelengths and the effect of a faulty choice of the two wavelengths in the two-wavelength cytophotometric method of Patau and Ornstein are studied by means of a computer simulation and in practice upon pararosaniline-Schiff stained nuclei. A simplification of the measuring strategy is proposed without affecting the accuracy.
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