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. 2021 Jan-Mar;22(1):16-22.
doi: 10.18502/jri.v22i1.4991.

Inhibition of Fatty Acid Binding Protein 4 in Obese Male Mice Adversely Affects Reproductive Parameters

Tevfik Balci  1 , Rahim Kocabas  2 , Gokhan Cuce  3 , Mehmet Akoz  4
Affiliations

Inhibition of Fatty Acid Binding Protein 4 in Obese Male Mice Adversely Affects Reproductive Parameters

Tevfik Balci et al. J Reprod Infertil. 2021 Jan-Mar.

Abstract

Background: As obesity is increasing worldwide, obese people use various methods to get rid of excess weight. BMS309403 (A drug) is a specific inhibitor of fatty acid binding protein 4. In this study, the effects of the BMS309403 on serum biochemical markers, testis tissue spermatogenesis and apoptotic markers were investigated in male mice.

Methods: Balb/c mice (total=56, each group n=14) were divided into control, obese control, obese solvent and obese drug groups. The obese control, obese solvent and obese drug groups were fed on the high sucrose diet to lead to obesity. After the development of obesity, BMS309403 was orally administered to the obese drug group for six weeks. It was performed in testicular tissues (Johnson Score and apoptosis markers) and biochemical tests (total testosterone, sex hormone binding globulin, inhibin-B tests and free androgen index) were used to evaluate reproductive parameters. The p<0.05 was considered to indicate a statistical significance.

Results: Serum fatty acid binding protein 4 levels were higher in obese control group and obese solvent group, compared to control (p<0.05) and obese drug groups (p<0.001). Serum total testosterone, free androgen index, inhibin-B, sex hormone binding globulin levels, testicular tissue B-cell lymphoma-2 expression level and Johnson Score parameters were lower in all obese groups compared with the control group. Inhibin-B levels and Johnson Score results were lower in obese drug group compared to other two obese groups (p<0.05).

Conclusion: Contrary to expectations, the use of BMS309403 negatively affected male reproductive parameters. Negative changes in reproductive parameters may be a result of the increased lee index of obesity.

Keywords: BMS309403; Experimental obesity; Fatty acid binding protein 4; Male fertility impairment; Mice; Side-effects.

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Conflict of interest statement

Conflict of Interest Authors declare no conflict of interest.

Figures

Figure 1.
Figure 1.
BAX expressions in the testis tissue; A) Negatif expression, B) +1 expression, C) +2 expression, D) +3 expression. BCL-2 expressions in the testis tissue; A) +3 expression, B) Negatif expression, C) +1 expression, D) +1 expression
Figure 2.
Figure 2.
Representative testis sections stained in the TUNEL assay; arrows indicated TUNEL-positive cells. Representative hematoxylin–eosin (H&E)-stained testis sections quantitatively assessed through Johnson Score for spermatogenesis and germ cell health
See this image and copyright information in PMC

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