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. 2021 Feb 19:2021:6678901.
doi: 10.1155/2021/6678901. eCollection 2021.

Quantitative Analysis of Colistin-Resistant Escherichia coli in Retail Meat from Local Vietnamese Markets

Thang N Nguyen  1 , Diep T Khong  1 , Ha V Le  1 , Hoa T Tran  1 , Quang N Phan  1 , Huong T T Le  1 , Ryuji Kawahara  2 , Yoshimasa Yamamoto  3
Affiliations

Quantitative Analysis of Colistin-Resistant Escherichia coli in Retail Meat from Local Vietnamese Markets

Thang N Nguyen et al. Biomed Res Int. .

Abstract

The spread of drug-resistant bacteria via food has contributed to the dissemination of resistant bacteria among humans. However, the status of food contamination with resistant bacteria, particularly the quantitative level of resistant bacteria in food, has not yet been well elucidated. In this study, the abundance of colistin-resistant Escherichia coli in meat samples was quantified to understand the origin of the contamination of meat available in local Vietnamese markets. Fifteen samples each of chicken and pork meat purchased from local Vietnamese markets were assessed for the presence of colistin-resistant E. coli with the mobile colistin resistance gene, mcr. The results showed that 40% (6/15) and 66% (10/15) of the pork and chicken meat samples, respectively, were contaminated with colistin-resistant E. coli. The median quantitative levels of colistin-resistant E. coli in the contaminated pork and chicken samples were 1.8 × 104 and 4.2 × 103 CFU/g, respectively. The results of phylogenetic analysis of isolates from a chicken meat sample showed that the contaminated colistin-resistant E. coli was a mix of multiple phylogenetical clones of bacteria that may have multiplied during sale. This is the first study to quantify the abundance of colistin-resistant E. coli in meat samples.

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Conflict of interest statement

The authors declare that there is no conflict of interest.

Figures

Figure 1
Figure 1
Levels of Escherichia coli-like and colistin-resistant E. coli-like bacteria from individual samples. (a) Pork meat samples; (b) chicken meat samples. ECC indicates the level of E. coli-like bacteria. COL-APSE indicates the level of colistin-resistant (CR) E. coli-like bacteria. See Materials and Methods for the quantification of bacteria in samples.
Figure 2
Figure 2
Dendrogram of pulsed-field gel patterns among colistin-resistant Escherichia coli colonies. A dendrogram of pulsed-field gel patterns among colistin-resistant Escherichia coli colonies on a CHROMagar COL-APSE agar plate cultured with a chicken meat sample homogenate was constructed. Strains from six randomly selected colonies from the plate were assessed.
See this image and copyright information in PMC

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