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. 2021 Jul;76(7):2288-2291.
doi: 10.1111/all.14808. Epub 2021 Mar 24.

Transcriptome programming of IL-3-dependent bone marrow-derived cultured mast cells by stem cell factor (SCF)

Ying Wang  1 , Kazufumi Matsushita  1   2 , Jennifer Jackson  1 , Takafumi Numata  1 , Yue Zhang  3 , Gao Zhou  3 , Mindy Tsai  1   2 , Stephen J Galli  1   2   4
Affiliations

Transcriptome programming of IL-3-dependent bone marrow-derived cultured mast cells by stem cell factor (SCF)

Ying Wang et al. Allergy. 2021 Jul.
No abstract available

Keywords: IgE; basic immunology; mast cell; stem cell factor; transcriptome.

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Figures

Figure 1
Figure 1
Transcriptome profiling of peritoneal mast cells (PMCs) vs. cultured SCF-MCs and IL-3-MCs. (A) Population principal component analysis (PCA). (B) MC population hierarchical clustering (top margin). (C-E) Heatmaps of MC-enriched protease-encoding transcripts (C), MAS related GPR (MRGPR) family-encoding transcripts (D) and bioamine biosynthesis transcripts (E). Genes are ordered by log2 fold changes between PMC and IL-3-MCs, and DE genes between PMC and. IL-3-MCs with an adjusted P value <0.05 are in red. Data: PMCs from four mice vs. IL-3- and SCF-MCs from two mice.
Figure 2
Figure 2
Mrgprb2 transcript upregulation in SCF-MCs is associated with enhanced responsiveness to substance P (SP). (A) Heatmaps and (B) RT-qPCR of Mrgpr-transcripts. Data presented as relative expression of transcripts vs. β-actin. Data represent MCs derived from two mice for RT-qPCR. * P<0.05 between the two groups. (C) Substance P (SP) or (D) compound 48/80 activation of IL-3- or SCF-MCs quantified as mean fluorescence intensity (MFI) of Alexa 488-conjugated avidin (details in online Methods). (E) Histamine release; stimulation for 1 h. * P <0.05 at certain time points or with certain drug concentrations for 30 min. Error bars represent SEM.
See this image and copyright information in PMC

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