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. 2021 Apr 15;384(15):1466-1468.
doi: 10.1056/NEJMc2102017. Epub 2021 Mar 8.

Neutralizing Activity of BNT162b2-Elicited Serum

Yang Liu  1 , Jianying Liu  1 , Hongjie Xia  1 , Xianwen Zhang  1 , Camila R Fontes-Garfias  1 , Kena A Swanson  2 , Hui Cai  2 , Ritu Sarkar  2 , Wei Chen  2 , Mark Cutler  2 , David Cooper  2 , Scott C Weaver  1 , Alexander Muik  3 , Ugur Sahin  3 , Kathrin U Jansen  2 , Xuping Xie  4 , Philip R Dormitzer  5 , Pei-Yong Shi  6
Affiliations

Neutralizing Activity of BNT162b2-Elicited Serum

Yang Liu et al. N Engl J Med. .
No abstract available

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Figures

Figure 1
Figure 1. Serum Neutralization of Variant Strains of SARS-CoV-2 after the Second Dose of BNT162b2 Vaccine.
Shown are the results of 50% plaque reduction neutralization testing (PRNT50) with the use of 20 samples obtained from 15 trial participants 2 weeks (circles) or 4 weeks (triangles) after the administration of the second dose of the BNT162b2 vaccine. The mutant viruses were obtained by engineering the full set of mutations in the B.1.1.7, P.1., or B.1.351 lineage or subsets of the S gene mutations in the B.1.351 lineage (B.1.351-Δ242-244+D614G and B.1.351-RBD+D614G) into USA-WA1/2020. Each data point represents the geometric mean PRNT50 obtained with a serum sample against the indicated virus, including data from repeat experiments, as detailed in Table S1 in the Supplementary Appendix. The data for USA-WA1/2020 are from three experiments; for B.1.1.7-spike, B.1.351-Δ242-244+D614G, and B.1.351-RBD-D614G viruses from one experiment each; and for P.1-spike and B.1.351-spike viruses from two experiments each. In each experiment, the neutralization titer was determined in duplicate assays, and the geometric mean was taken. The heights of bars and the numbers over the bars indicate geometric mean titers. The 𝙸 bars indicate 95% confidence intervals. Statistical analysis was performed with the use of the Wilcoxon signed-rank test. The statistical significance of the difference between geometric mean titers in the USA-WA1/2020 neutralization assay and in each variant virus neutralization assay with the same serum samples are as follows: P=0.02 for B.1.1.7-spike; P=0.06 for P.1-spike; P<0.001 for B.1.351-spike; P=0.99 for B.1.351-Δ242-244+D614G; and P=0.005 for B.1.351-RBD+D614G. LOD denotes limit of detection.
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