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. 2021 Jul;134(4):653-664.
doi: 10.1007/s10265-021-01276-6. Epub 2021 Mar 8.

Screening of mutants using chlorophyll fluorescence

Takako Ogawa  1 , Kintake Sonoike  2
Affiliations

Screening of mutants using chlorophyll fluorescence

Takako Ogawa et al. J Plant Res. 2021 Jul.

Abstract

Chlorophyll fluorescence has been widely used for the estimation of photosynthesis or its regulatory mechanisms. Chlorophyll fluorescence measurements are the methods with non-destructive nature and do not require contact between plant materials and fluorometers. Furthermore, the measuring process is very rapid. These characteristics of chlorophyll fluorescence measurements make them a suitable tool to screen mutants of photosynthesis-related genes. Furthermore, it has been shown that genes with a wide range of functions can be also analyzed by chlorophyll fluorescence through metabolic interactions. In this short review, we would like to first introduce the basic principle of the chlorophyll fluorescence measurements, and then explore the advantages and limitation of various screening methods. The emphasis is on the possibility of chlorophyll fluorescence measurements to screen mutants defective in metabolisms other than photosynthesis.

Keywords: Chlorophyll fluorescence; Imaging; Kautsky transient; Metabolic interaction; OJIP analysis; Screening.

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References

    1. Aoki M, Katoh S (1982) Oxidation and reduction of plastoquinone by photosynthetic and respiratory electron transport in a cyanobacterium Synechococcus sp. Biochem Biophys Acta 682:307–314
    1. Badger MR, Fallahi H, Kaines S, Takahashi S (2009) Chlorophyll fluorescence screening of Arabidopsis thaliana for CO2 sensitive photorespiration and photoinhibition mutants. Funct Plant Biol 36:867–873 - PubMed
    1. Barbagallo RP, Oxborough K, Pallett KE, Baker NR (2003) Rapid, noninvasive screening for perturbations of metabolism and plant growth using chlorophyll fluorescence imaging. Plant Physiol 132:485–493 - PubMed - PMC
    1. Battchikova N, Eisenhut M, Aro E-M (2011a) Cyanobacterial NDH-1 complexes: novel insights and remaining puzzles. Biochim Biophys Acta 1807:935–944 - PubMed
    1. Battchikova N, Wei L, Du L, Bersanini L, Aro E-M, Ma W (2011b) Identification of novel Ssl0352 protein (NdhS), essential for efficient operation of cyclic electron transport around photosystem I, in NADPH:plastoquinone oxidoreductase (NDH-1) complexes of Synechocystis sp. PCC 6803. J Biol Chem 286:36992–37001 - PubMed - PMC

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