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. 2021 Mar;26(4):043008.
doi: 10.1117/1.JBO.26.4.043008.

Terahertz high-resolution spectroscopy of thermal decomposition gas products of diabetic and non-diabetic blood plasma and kidney tissue pellets

Anastasiya A Lykina  1 , Vladimir A Anfertev  2 , Elena G Domracheva  2 , Mariya B Chernyaeva  2   3 , Yulia A Kononova  4 , Yana G Toropova  4 , Dmitry V Korolev  4 , Olga A Smolyanskaya  1 , Vladimir L Vaks  2
Affiliations

Terahertz high-resolution spectroscopy of thermal decomposition gas products of diabetic and non-diabetic blood plasma and kidney tissue pellets

Anastasiya A Lykina et al. J Biomed Opt. 2021 Mar.

Abstract

Significance: One of the modern trends in medical diagnostics is based on metabolomics, an approach allowing determination of metabolites which can be the specific features of disease. High-resolution gas spectroscopy allows investigation of the gas metabolite content of samples of biological origin. We present the elaboration of a method of studying diabetic and non-diabetic biological samples, prepared as pellets, by terahertz (THz) high-resolution spectroscopy.

Aim: The main idea of the work is studying the content of thermal decomposition gas products of diabetic and non-diabetic dried blood plasma and kidney tissues for revealing the set of gas-markers that characterized the diabetes by the THz high-resolution spectroscopy method.

Approach: We present an approach to study the diabetic and non-diabetic blood plasma (human and rats) and kidney tissues (rats), using high-resolution spectroscopy based on the non-stationary effect of THz frequency range. The methods of preparing the blood and kidney tissue samples as pellets and of vaporizing the samples were developed.

Results: The measurements of rotational absorption spectra of vapors at heating the pellets prepared from blood and kidney tissue were carried out in 118 to 178 GHz frequency range. The absorption lines appearing in spectra of the sample vapors were detected and identified. The molecular contents of thermal decomposition products differed for non-diabetic and diabetic samples; e.g., main marker is acetone appearing in the diabetic blood (human and rats) and in the diabetic kidney tissue.

Conclusions: Our paper illustrates the potential ability for determining the metabolite content of biological samples for diagnostics and prognosis of diseases for clinical medicine.

Keywords: blood plasma; diabetes; high-resolution terahertz spectroscopy; lyophilization; thermal decomposition; tissue.

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Figures

Fig. 1
Fig. 1
Recording parts of the absorption spectra with a line of acetone (f=138.28528  GHz, lgI=−5.5867, 38 28 10 0 ← 38 27 11 1) in a sample of diabetic human blood plasma in THz spectrometer operating range.
Fig. 2
Fig. 2
The record of formic acid absorption line (f=133.7671873  GHz, lgI=−3.8183 (6 0 6 ←5 0 5) in the sample of diabetic rats’ kidneys tissue.
Fig. 3
Fig. 3
The record of methanethiol absorption lines (f1=126.4038344  GHz, lgI1=−4.4357, 5 0 5 1 ← 4 0 4 1 v=0 to 2 and f2=126.4056761  GHz, lgI2=−4.4339, 5 0 5 0 ← 4 0 4 0 v=0 to 2) in the sample of diabetic rats’ kidneys tissue.
See this image and copyright information in PMC

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