Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2021:2269:245-254.
doi: 10.1007/978-1-0716-1225-5_17.

Engineered Tissues Made from Human iPSC-Derived Schwann Cells for Investigating Peripheral Nerve Regeneration In Vitro

Rebecca Powell  1 , James B Phillips  2
Affiliations

Engineered Tissues Made from Human iPSC-Derived Schwann Cells for Investigating Peripheral Nerve Regeneration In Vitro

Rebecca Powell et al. Methods Mol Biol. 2021.

Abstract

Peripheral nerves have a limited ability to regenerate and current clinical approaches involving microsurgery give suboptimal recovery. Engineered tissues using aligned cellular collagen hydrogels can be used as in vitro models through the incorporation of human Schwann cells. However, primary human Schwann cells are difficult to obtain and can be challenging to culture. The ability to generate Schwann cells from human-induced pluripotent stem cells (hiPSCs) provides a more reliable cell source for modeling peripheral nerve tissue. Here, we describe protocols for generating hiPSC-derived Schwann cells and incorporating them into 3D engineered tissue culture models for peripheral nerve research.

Keywords: 3D culture model; Hydrogel; Peripheral nerve; Pluripotent stem cell; Regeneration; Schwann cell; Tissue engineering.

PubMed Disclaimer

References

    1. Huang CW, Huang WC, Qiu X et al (2017) The differentiation stage of transplanted stem cells modulates nerve regeneration. Sci Rep 7(1):17401. https://doi.org/10.1038/s41598-017-17043-4 - DOI - PubMed - PMC
    1. Kim HS, Lee J, Lee DY et al (2017) Schwann cell precursors from human pluripotent stem cells as a potential therapeutic target for myelin repair. Stem Cell Rep 8(6):1714–1726. https://doi.org/10.1016/j.stemcr.2017.04.011 - DOI
    1. Phillips JB, Brown R (2011) Micro-structured materials and mechanical cues in 3D collagen gels. Methods Mol Biol 695:183–196. https://doi.org/10.1007/978-1-60761-984-0_12 - DOI - PubMed
    1. O’Rourke C, Lee-Reeves C, Drake RA et al (2017) Adapting tissue-engineered in vitro CNS models for high-throughput study of neurodegeneration. J Tissue Eng 8:2041731417697920. https://doi.org/10.1177/2041731417697920 - DOI - PubMed - PMC
    1. Georgiou M, Bunting SC, Davies HA et al (2013) Engineered neural tissue for peripheral nerve repair. Biomaterials 34(30):7335–7343. https://doi.org/10.1016/j.biomaterials.2013.06.025 - DOI - PubMed

Publication types

MeSH terms

LinkOut - more resources