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. 2021:2269:255-268.
doi: 10.1007/978-1-0716-1225-5_18.

Modulation of a Stem Cell Gene: LGR4 Knockout in a Human Cell Line by CRISPR/Cas Method

Swetlana Rot  1 , Matthias Kappler  2
Affiliations

Modulation of a Stem Cell Gene: LGR4 Knockout in a Human Cell Line by CRISPR/Cas Method

Swetlana Rot et al. Methods Mol Biol. 2021.

Abstract

The modulation of gene expression is essential for the investigation of function or involved pathway of a single gene of interest, in particular in the developmental/stem cell biology. The temporary knock down of gene expression via siRNA is a well-established but with a residual expression connected modulation method. The chapter describes the complete knockout of a defined target and allows a comprehensive study of different gene like the stem cell gene LGR4 (Leucine-rich repeat-containing G-protein-coupled receptor 4) using the new developed CRISPR/Cas method (clustered regularly interspaced short palindromic repeats).

Keywords: CRISPR/Cas; Genome editing; Knock down; LGR4; Stem cell gene.

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