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. 2021:2259:205-213.
doi: 10.1007/978-1-0716-1178-4_13.

Shotgun Proteomics for Food Microorganism Detection

Ana G Abril  1 , Ignacio Ortea  2 , Jorge Barros-Velázquez  3 , Tomás G Villa  1 , Pilar Calo-Mata  4
Affiliations

Shotgun Proteomics for Food Microorganism Detection

Ana G Abril et al. Methods Mol Biol. 2021.

Abstract

Classical and culture-based methods for the identification and characterization of the biochemical properties of microorganisms are slow and labor-intensive. Liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) has been used for the analysis of bacterial pathogen strain-specific diagnostic peptides allowing the characterization of bacterial strains.Here, we describe the analysis of tryptic digestion peptides by LC-ESI-MS/MS to search for specific biomarkers useful for the rapid identification of, on the one hand, the bacterial species and, on the other hand, the physiological and biochemical characteristics such as the expression of virulence factors, including toxins, immune-modulatory factors, and exoenzymes.

Keywords: LC–ESI-MS/MS; Mass spectrometry; Microorganism detection; Peptide biomarker.

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