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. 2022 Jan;200(1):318-329.
doi: 10.1007/s12011-021-02628-y. Epub 2021 Mar 11.

Astragalus Polysaccharide Protects Against Cadmium-Induced Autophagy Injury Through Reactive Oxygen Species (ROS) Pathway in Chicken Embryo Fibroblast

Xudong Shen  1   2 , Zequn Tang  1   2 , Yu Bai  3 , Meishuo Wan  1   2 , Miao Yu  1   2 , Jingyi Chen  1   2 , Guangxing Li  1   2 , Ruili Zhang  4   5 , Ming Ge  1   2
Affiliations

Astragalus Polysaccharide Protects Against Cadmium-Induced Autophagy Injury Through Reactive Oxygen Species (ROS) Pathway in Chicken Embryo Fibroblast

Xudong Shen et al. Biol Trace Elem Res. 2022 Jan.

Abstract

Cadmium (Cd) is a harmful heavy metal pollutant, which can cause oxidative stress in the body and induce cell damage. Reactive oxygen species (ROS) is a general term for substances that contain oxygen and are active in the body. However, excessive ROS can damage the body. Cadmium poisoning can cause a large amount of ROS in cells and autophagy. Astragalus polysaccharide (APS) is a plant polysaccharide with biological functions, such as antioxidant and anti-stress activities. In this study, chicken embryo fibroblasts (CEF) were used to determine the relationship between ROS and autophagy damage of Cd-infected cells and the mechanism of APS on cadmium-induced autophagy damage. The results showed that a 10-μL dose of 10 μmol/L cadmium chloride (CdCl2) can induce CEF autophagy and damage when CEF was added for 36 h. Cadmium induced CEF autophagy damage by increasing ROS production. APS could significantly reduce ROS production and LC3-II and Beclin-1 protein expression, increase the expression of mTOR and the level of antioxidation, and restore the viability and morphological damage of CEF exposed to Cd. Our study suggests that APS can alleviate Cd-induced CEF autophagy damage by reducing the production of ROS.

Keywords: Astragalus polysaccharide; Autophagy; Cadmium; Chicken embryo fibroblasts; Reactive oxygen species.

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