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. 2021:2291:285-296.
doi: 10.1007/978-1-0716-1339-9_13.

Human Epithelial Stem Cell-Derived Colonoid Monolayers as a Model to Study Shiga Toxin-Producing Escherichia coli-Host Interactions

Karol Dokladny  1 , Julie G In  1 , James Kaper  2 , Olga Kovbasnjuk  3
Affiliations

Human Epithelial Stem Cell-Derived Colonoid Monolayers as a Model to Study Shiga Toxin-Producing Escherichia coli-Host Interactions

Karol Dokladny et al. Methods Mol Biol. 2021.

Abstract

Human intestinal organoid cultures established from crypt-derived stem cells truly revolutionized our approach to study intestinal epithelial physiology and pathologies as they can be propagated indefinitely and preserve the genetic signature of the donor and the gut segment specificity in culture. Here we describe human stem cell-derived colonoid monolayers as a reliable and reproducible model to study Shiga toxin-producing Escherichia coli (STEC) infection and STEC-caused pathologies of the whole colonic epithelium comprising a mixture of colonocytes, goblet, enteroendocrine, and other rare cells present in human colonic epithelial tissue.

Keywords: Colonoid monolayers; Human intestinal organoids; STEC; Shiga toxin; Stem cells.

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