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. 2021 Feb 24:2021:6667238.
doi: 10.1155/2021/6667238. eCollection 2021.

Identification of Chemical Composition and Metal Determination of Retama raetam (Forssk) Stem Constituents Using ICP-MS, GC-MS-MS, and DART-MS

Wedad Al-Onazi  1 , Amal M Al-Mohaimeed  1 , Musarat Amina  2 , Maha F El-Tohamy  1
Affiliations

Identification of Chemical Composition and Metal Determination of Retama raetam (Forssk) Stem Constituents Using ICP-MS, GC-MS-MS, and DART-MS

Wedad Al-Onazi et al. J Anal Methods Chem. .

Abstract

This study aims to investigate the chemical constituents of the stem of Retama raetam growing in Saudi Arabia. The organic and inorganic composition of ethanol extract of R. raetam stem has been explored using direct analysis in real time-mass spectrometry (DART-MS), gas chromatography-mass spectrometry (GC-MS), and inductively coupled plasma-mass spectrometry (ICP-MS). Analysis conducted by DART-MS and GC-MS reveals the presence of several interesting organic constituents identified as 2,4-di-tert-butylphenol, sparteine, benzenepropanoic acid, 3,5-bis(1,1-dimethylethyl)-4-hydroxy-, methyl ester, phthalic acid, 1-octadecanol, squalene, argentamin, 2,4-di-tert-butylphenol, sparteine, benzene propanoic acid, 3,5-bis(1,1-dimethylethyl)-4-hydroxy-, methyl ester, phthalic acid, 1-octadecanol, squalene, argentamin, and hentriacontane in the ethanol extract of the R. raetam stem. The ICP-MS analysis of stem extract showed the presence of a significant amount of important inorganic elements including aluminum, chlorine, calcium, bromine magnesium, phosphorus, scandium, and chromium. The current study complements other R. raetam extract investigations carried out in the past and provides the additional data for the future research studies.

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Conflict of interest statement

The authors declare that they have no conflicts of interest.

Figures

Figure 1
Figure 1
Main constituents characterized in R. raetam plant stem by DART-MS.
Figure 2
Figure 2
GC-MS-MS chromatogram of ethanol stem extract of R. raetam.
Figure 3
Figure 3
Results of DPPH radical scavenging potential of the R. raetam stem in comparison with ascorbic acid as reference.
See this image and copyright information in PMC

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