Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1977 Oct 20;155(2):213-7.
doi: 10.1007/BF00393162.

Transformation of Escherichia coli by a specific DNA restriction fragment

S M Schweitzer, H Matzura

Transformation of Escherichia coli by a specific DNA restriction fragment

S M Schweitzer et al. Mol Gen Genet. .

Abstract

Specific transformation of a rifampicin sensitive strain of Escherichia coli to rifampicin resistance has been performed by a single, defined DNA restriction fragment carrying the genetic information for the beta subunit of E. coli RNA polymerase. In this transformation the transforming genetic character has been substituted for the corresponding recipient gene locus by recombination. The value of the described transformation system for locating genetic markers on DNA restriction fragments is discussed in comparison to previously reported in vitro systems.

PubMed Disclaimer

References

    1. Proc Natl Acad Sci U S A. 1973 Nov;70(11):3240-4 - PubMed
    1. Proc Natl Acad Sci U S A. 1969 Apr;62(4):1159-66 - PubMed
    1. FEBS Lett. 1970 Dec;11(3):165-168 - PubMed
    1. Eur J Biochem. 1976 Dec;71(1):19-24 - PubMed
    1. J Biol Chem. 1969 Nov 25;244(22):6160-7 - PubMed