doi: 10.1093/nar/4.10.3599.
An NMR study of the exchange rates for protons involved in the secondary and tertiary structure of yeast tRNA Phe
- PMID: 337239
- PMCID: PMC342676
- DOI: 10.1093/nar/4.10.3599
Item in Clipboard
An NMR study of the exchange rates for protons involved in the secondary and tertiary structure of yeast tRNA Phe
Nucleic Acids Res.
1977 Oct.
Free PMC article
Abstract
Solvent exchange rates of all the protons of yeast tRNAphe resonating in the lowfield NMR region (-11 to-15 ppm from DSS) have been measured by saturation-recovery long-pulse Fourier transform NMR. All these protons in yeast tRNAphe are in the fast exchange limit with H2O relative to their intrinsic longitudinal relaxation processes. Most rates show very little temperature dependence; however, tertiary base pair protons are preferentially destabilized in the absence of Mg++ at higher temperatures. The measured exchange rates are between 2 and 125 sec-1 for a temperature range from 10 degrees C to 45 degrees C and MgCl2 concentrations between 0 and 15 mM.
Similar articles
-
Real-time solvent exchange studies of the imino and amino protons of yeast phenylalanine transfer RNA by Fourier transform NMR.Proc Natl Acad Sci U S A. 1979 Jul;76(7):3130-4. doi: 10.1073/pnas.76.7.3130. Proc Natl Acad Sci U S A. 1979. PMID: 386331 Free PMC article.
-
Pulsed FT-NMR double resonance studies of yeast tRNAPhe: specific nuclear Overhauser effects and reinterpretation of low temperature relaxation data.Nucleic Acids Res. 1978 Oct;5(10):3913-27. doi: 10.1093/nar/5.10.3913. Nucleic Acids Res. 1978. PMID: 364421 Free PMC article.
-
Proton exchange and basepair kinetics of yeast tRNA(Phe) and tRNA(Asp1).J Biochem. 1995 Mar;117(3):515-20. doi: 10.1093/oxfordjournals.jbchem.a124738. J Biochem. 1995. PMID: 7629016
-
High-resolution nuclear magnetic resonance investigations of the structure of tRNA in solution.Prog Nucleic Acid Res Mol Biol. 1976;18:91-149. doi: 10.1016/s0079-6603(08)60587-5. Prog Nucleic Acid Res Mol Biol. 1976. PMID: 790475 Review. No abstract available.
-
Three-dimensional structure of transfer RNA.Prog Nucleic Acid Res Mol Biol. 1976;17:181-216. doi: 10.1016/s0079-6603(08)60070-7. Prog Nucleic Acid Res Mol Biol. 1976. PMID: 778921 Review. No abstract available.
Cited by
-
Proton exchange rates in transfer RNA as a function of spermidine and magnesium.Nucleic Acids Res. 1983 Apr 11;11(7):2121-34. doi: 10.1093/nar/11.7.2121. Nucleic Acids Res. 1983. PMID: 6340067 Free PMC article.
-
Nuclear Overhauser effect study and assignment of D stem and reverse-Hoogsteen base pair proton resonances in yeast tRNAAsp.Nucleic Acids Res. 1981 Dec 21;9(24):7073-83. doi: 10.1093/nar/9.24.7073. Nucleic Acids Res. 1981. PMID: 6278454 Free PMC article.
-
Correlation of lac operator DNA imino proton exchange kinetics with its function.Proc Natl Acad Sci U S A. 1984 Jun;81(12):3665-9. doi: 10.1073/pnas.81.12.3665. Proc Natl Acad Sci U S A. 1984. PMID: 6328523 Free PMC article.
-
Sequence dependence of hydrogen exchange kinetics in DNA duplexes at the individual base pair level in solution.Proc Natl Acad Sci U S A. 1983 Apr;80(8):2184-8. doi: 10.1073/pnas.80.8.2184. Proc Natl Acad Sci U S A. 1983. PMID: 6572970 Free PMC article.
-
A role for the MS analysis of nucleic acids in the post-genomics age.J Am Soc Mass Spectrom. 2010 Jan;21(1):1-13. doi: 10.1016/j.jasms.2009.09.006. Epub 2009 Sep 17. J Am Soc Mass Spectrom. 2010. PMID: 19897384
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
