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. 2021 Jul;32(5):1125-1129.
doi: 10.1111/pai.13504. Epub 2021 Apr 18.

SARS-CoV-2-specific IgG1/IgG3 but not IgM in children with Pediatric Inflammatory Multi-System Syndrome

Marisol Perez-Toledo  1 , Sian E Faustini  1 , Sian E Jossi  1 , Adrian M Shields  1 , Edith Marcial-Juarez  1 , Hari Krishnan Kanthimathinathan  2   3 , Joel D Allen  4 , Yasunori Watanabe  5 , Margaret Goodall  1 , Benjamin E Willcox  1 , Carrie R Willcox  1 , Mahboob Salim  1 , David C Wraith  1 , Tonny V Veenith  6 , Eleni Syrimi  1 , Mark T Drayson  1 , Deepthi Jyothish  7 , Eslam Al-Abadi  2   8 , Ashish Chikermane  9 , Steven B Welch  10 , Kavitha Masilamani  7 , Scott Hackett  11 , Max Crispin  4 , Barnaby R Scholefield  3   12 , Adam F Cunningham  1 , Alex G Richter  1
Affiliations

SARS-CoV-2-specific IgG1/IgG3 but not IgM in children with Pediatric Inflammatory Multi-System Syndrome

Marisol Perez-Toledo et al. Pediatr Allergy Immunol. 2021 Jul.
No abstract available

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Conflict of interest statement

MTD and MG have a commercial relationship with Abingdon Health. The rest of the authors declared no conflict of interest. AFC is grateful for funding from The Medical Research Council (MC_PC_17183), the Global Challenges Research Fund (GCRF), and The Institute for Global Innovation (IGI, Project 3107) of The University of Birmingham. This study was supported by the UK National Institute for Health Research, Birmingham Biomedical Research Centres Funding scheme. Dr Barnaby Scholefield is funded by the NIHR Clinician Scientist fellowship program. The work in Prof. Max Crispin's laboratory was funded by the International AIDS Vaccine Initiative, Bill and Melinda Gates Foundation through the Collaboration for AIDS Vaccine Discovery (OPP1084519 and OPP1115782), the Scripps Consortium for HIV Vaccine Development (CHAVD) (AI144462), and the University of Southampton Coronavirus Response Fund which has over 1000 donors from around the world.

Figures

FIGURE 1
FIGURE 1
Detection of antibodies against spike glycoprotein and nucleocapsid from SARS‐CoV‐2 in children with PIMS‐TS. Serological responses were detected against purified near‐full‐length trimeric SARS‐CoV‐2 viral spike glycoprotein (S) or nucleocapsid (N) by ELISA. A, Optical density at 450 nm (OD450) of individual sera at a single dilution (1:40) from pre‐2019 healthy adult donors (green), sera from children with PIMS‐TS (red), or plasma from adult patients in intensive therapy unit (ITU, yellow) detected using combined anti‐IgG, IgA, and IgM. One symbol represents results for a single serum, and the bar shows the median values for each group. B‐F, Area under the curve (AUC) for individual sera against S glycoprotein and N from pre‐2019 negative control donors (green), children with PIMS‐TS (red), or plasma from adult ITU patients (yellow) serially diluted fivefold from 1:20 or 1:50; primary antibodies were detected with (B) anti‐IgM, (C) anti‐IgG, (D) anti‐IgA, (E) anti‐IgG1, or (F) anti‐IgG3. Each point represents one individual; horizontal line represents the mean ± SD. Two‐tailed one‐way ANOVA with Holm‐Sidak's test post hoc was applied to calculate statistical differences. *P <.05, **P <.005, ***P <.001, ****P <.0001, ns, non‐significant
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References

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