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. 2021 Feb;170(4):410-414.
doi: 10.1007/s10517-021-05077-8. Epub 2021 Mar 16.

Perfluorocarbon Protects against Lipopolysaccharide-Induced Apoptosis of Endothelial Cells in Pulmonary Microvessels

Y Xu  1 , T Wu  2 , P Wang  1 , Z X Liang  1 , S S Shi  2 , S F Xu  3 , X J Liu  2 , Q Tian  2
Affiliations

Perfluorocarbon Protects against Lipopolysaccharide-Induced Apoptosis of Endothelial Cells in Pulmonary Microvessels

Y Xu et al. Bull Exp Biol Med. 2021 Feb.

Abstract

This study was aimed to explore the effect and mechanisms of action of perfluorocarbon on LPS-induced apoptosis of pulmonary microvascular endothelial cells (PMVEC) isolated from Sprague-Dawley rats. Apoptosis rates were assessed by flow cytometry. Ultrastructural characteristics of PMVEC were evaluated by transmission electron microscopy. The protein expression of cleaved caspase-3 was measured using Western blotting. LPS significantly increased the level of apoptosis, induced the appearance of ultrastructural changes typical of apoptosis, up-regulated the expression of active caspase-3 protein. These effects of LPS were attenuated by co-administration of perfluorocarbon. These results suggest that perfluorocarbon can attenuate LPS-induced apoptosis of PMVEC by inhibiting TLR-4 signaling and caspase-3 activation.

Keywords: apoptosis; caspase-3; lipopolysaccharide; perfluorocarbon; pulmonary microvascular endothelial cells.

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