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. 2021 May;114(5):507-513.
doi: 10.1007/s10482-021-01536-4. Epub 2021 Mar 17.

Anaerocolumna sedimenticola sp. nov., isolated from fresh water sediment

Juseok Kim  1   2 , Joon Yong Kim  1 , Hye Seon Song  1 , Yeon Bee Kim  1 , Tae Woong Whon  1 , Seung Woo Ahn  1   2 , Se Hee Lee  1 , SeungRan Yoo  1 , Yu Jin Kim  1 , Jinjong Myoung  3 , Yoon-E Choi  2 , Hong-Seok Son  4 , Seong Woon Roh  5
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Anaerocolumna sedimenticola sp. nov., isolated from fresh water sediment

Juseok Kim et al. Antonie Van Leeuwenhoek. 2021 May.

Abstract

Strain CBA3638T was isolated from the Geum River sediment, Republic of Korea. The cells of strain CBA3638T were Gram-stain-positive, strictly anaerobic, rod-shaped, and 0.5-1.0 μm wide, and 4.0-4.5 μm long. Optimal growth occurred at 37 °C, pH 7.0, and 1.0% (w/v) NaCl. Based on the 16S rRNA gene sequence, the phylogenetic analysis showed that strain CBA3638T belongs to the genus Anaerocolumna in the family Lachnospiraceae, and is most closely related to Anaerocolumna cellulosilytica (94.6-95.0%). The DDH value with A. cellulosilytica SN021T showed 15.0% relatedness. The genome of strain CBA3638T consisted of one circular chromosome that is 5,500,435 bp long with a 36.7 mol% G + C content. The genome contained seven 16S-5S-23S rRNA operons and one antibiotic resistance-related transporter gene (mefA). Quinones were not detected. The predominant cellular fatty acids were C16:0 and C14:0 and the polar lipids were diphosphatidylglycerol, phosphatidylcholine, and uncharacterised polar lipids. Based on the polyphasic taxonomic analysis, we propose strain CBA3638T as a novel species in the genus Anaerocolumna, with the name Anaerocolumna sedimenticola sp. nov. The type strain is CBA3638T (= KACC 21652T = DSM 110663T).

Keywords: Anaerocolumna sedimenticola; Bacteria; New taxon; The Geum River.

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