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Observational Study
. 2021 Oct;40(10):2227-2234.
doi: 10.1007/s10096-021-04213-6. Epub 2021 Mar 17.

Impact of rapid multiplex PCR on management of antibiotic therapy in COVID-19-positive patients hospitalized in intensive care unit

Naouale Maataoui  1   2 , Lotfi Chemali  3 , Juliette Patrier  4 , Alexy Tran Dinh  5   6 , Lucie Le Fèvre  4 , Brice Lortat-Jacob  5 , Mehdi Marzouk  4 , Camille d'Humières  7   3 , Emilie Rondinaud  7   3 , Etienne Ruppé  7   3 , Philippe Montravers  5   6 , Jean-François Timsit  7   4 , Laurence Armand-Lefèvre  7   3
Affiliations
Observational Study

Impact of rapid multiplex PCR on management of antibiotic therapy in COVID-19-positive patients hospitalized in intensive care unit

Naouale Maataoui et al. Eur J Clin Microbiol Infect Dis. 2021 Oct.

Abstract

Because the diagnosis of co/superinfection in COVID-19 patients is challenging, empirical antibiotic therapy is frequently initiated until microbiological analysis results. We evaluated the performance and the impact of the BioFire® FilmArray® Pneumonia plus Panel on 112 respiratory samples from 67 COVID-19 ICU patients suspected of co/superinfections. Globally, the sensitivity and specificity of the test were 89.3% and 99.1%, respectively. Positive tests led to antibiotic initiation or adaptation in 15% of episodes and de-escalation in 4%. When negative, 28% of episodes remained antibiotic-free (14% no initiation, 14% withdrawal). Rapid multiplex PCRs can help to improve antibiotic stewardship by administering appropriate antibiotics earlier and avoiding unnecessary prescriptions.

Keywords: Antibiotic stewardship; COVID-19; Coinfection; Multiplex PCR; Superinfection.

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Conflict of interest statement

ERu received funds from bioMérieux and speaking fees from Mobidiag. JFT received lecture fees from bioMérieux and participates, outside of the submitted work, on the advisory boards of MSD, Pfizer, Bayer, Nabriva, Gilead, BD, 3M, Paratek. LA received speaking fees from bioMérieux.

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