Design of amino-functionalized hollow mesoporous silica cube for enzyme immobilization and its application in synthesis of phosphatidylserine

Abstract

In this study, hollow mesoporous silica cube (HMSC) modified with amino (-NH₂) were synthesized and applied in the immobilization of phospholipase D (PLD) via physical adsorption and chemical cross-linking strategy. The amino-functionalized nano carrier HMSC represented excellent immobilization ability and achieved 87.15 % immobilization rate. The immobilized PLD has wider pH application range and thermal stability, and maintained over 90% of the initial activity after incubation at 50 °C for 2 h. After 50 days of storage at 4 ℃, immobilized PLD retained 40.12 % of its initial activity while free PLD lost 88.28% of its initial activity. The modified HMSC with immobilized PLD (HMSC-NH₂-PLD) retained 50.73% activities after 9 consecutive reuses. Using the HMSC-NH₂-PLD, a high-efficient method for the conversion of phosphatidylserine (PS) from phosphatidylcholine (PC) and L-serine was proposed. The HMSC-NH₂-PLD exhibited prominent enzymatic activity for PS synthesis, the maximal conversion of PS was 90.40% with a catalytic efficiency (CE) of 31.95 μmol / (g h under the optimal conditions. The research in this paper provides a sustainable and efficient biocatalysis application for PS synthesis.

Keywords: Immobilization; Phospholipase; hosphatidylserine.