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. 2021 Mar;45(1):252-257.
doi: 10.1007/s12639-020-01302-9. Epub 2020 Nov 7.

Ovicidal and toxicological effect of hydroalcoholic extracts of Euphorbia milli var splendens, Synadenium carinatum Boiss and Tagetes minuta L. against Ancylostoma spp.: In vitro study

Matheus Diniz Gonçalves Coêlho  1   2 , Lucas Tobias Rodrigues Maciel  1 , Thaís de Fátima Kieko Ozaki  1 , Maria Eduarda Godoi Silva  1 , Lilian Saito Ormachea Bozo  1 , Yumi Ando Consoli  1 , Fernanda Bueno Sant'Anna Pereira-Maciel  1 , Gokithi Akisue  3 , Francine Alves da Silva-Coêlho  4
Affiliations

Ovicidal and toxicological effect of hydroalcoholic extracts of Euphorbia milli var splendens, Synadenium carinatum Boiss and Tagetes minuta L. against Ancylostoma spp.: In vitro study

Matheus Diniz Gonçalves Coêlho et al. J Parasit Dis. 2021 Mar.

Abstract

Nematodes of the Ancylostomidae family consist of important parasitic species. The control of such parasitosis represents an important challenge, given the constant high rate of reinfection of some hosts, among which the domestic dog, as well as the high environmental contamination. Another factor that can have a negative influence is the toxicity of the chemicals used for environmental decontamination, highlighting the need to design research to identify new control strategies for this parasitosis, among which the use of plant extracts. Thus, the objective of this research was to evaluate the toxicity and ovicidal activity of hydroalcoholic extracts obtained by percolation of three plants: Tagetes minuta L., Euphorbia milli var splendens (Bojer ex Hook.) Ursch & Leandri and Synadenium carinatum Boiss, against Ancylostoma spp. In addition, these extracts were tested at different concentrations for toxicity against Artemia salina L. and Allium cepa L. It was observed that the hydroalcoholic extract of all plant species evaluated induced moderate ovicide activity at all the tested concentration, with emphasis on E. milii, which was the only plant species that presented significantly low toxicity in the concentration of 12.5 µL/mL when compared to the other species evaluated.

Keywords: Ancylostoma spp; Environmental contamination; Plant extracts; Toxicity.

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Conflict of interest statement

Conflict of interestAll authors declare that: 1. The manuscript was not submitted to other journal; 2. The work is original; 3. There isn’t any plagiarism; 4. Results are presented without fabrication, falsification or inappropriate data manipulation. 5. All the results obtained are disposed in this publication, that is, it was not splitted out.

Figures

Fig. 1
Fig. 1
Evaluation of the toxicity of three plant extracts using the A. cepa L. test in relation to the average number of roots. * = significant difference in relation to the control group
Fig. 2
Fig. 2
Evaluation of the toxicity of three plant extracts using the A. cepa L. test in relation to the average root weight. * = significant difference in relation to the control group
Fig. 3
Fig. 3
Evaluation of the toxicity of three plant extracts using the A. cepa L. test in relation to the average weight gain of the bulbs. * = significant difference in relation to the control group
Fig. 4
Fig. 4
Evaluation of the toxicity of three plant extracts using the A. cepa L. test in relation to the average root size. * = significant difference in relation to the control group
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References

    1. Afonso-Cardoso SR, Rodrigues FH, Gomes MA, Silva AG, Rocha A, Guimaraes AH, Candeloro I, Favoreto S, Jr, Ferreira MS, de Souza MA. Protective effect of lectin from Synadenium carinatum on Leishmania amazonensis infection in BALB/c mice. Korean J Parasitol. 2007;45(4):255–266. doi: 10.3347/kjp.2007.45.4.255. - DOI - PMC - PubMed
    1. Araújo GL, Campos MAA, Valente MAS, Silva SCT, França FD, Chaves MM, Tagliati CA. Alternative methods in toxicity testing: the current approach. Braz J Pharm Sci. 2014;50(1):55–62. doi: 10.1590/S1984-82502011000100005. - DOI
    1. Cardoso MR, Mota CM, Ribeiro DP, Noleto PG, Andrade WB, Souza MA, Silva NM, Mineo TW, Mineo JR, Silva DA. Adjuvant and immunostimulatory effects of a D-galactose-binding lectin from Synadenium carinatum latex (ScLL) in the mouse model of vaccination against neosporosis. Vet Res. 2012;43(1):76. doi: 10.1186/1297-9716-43-76. - DOI - PMC - PubMed
    1. Ciappina AL, Ferreira FA, Pereira IR, Sousa TR, Matos FS, Melo-Reis PR, Gonçalves PJ, Bailão EFLC, Almeida LM. Toxicity of Jatropha curcas L. Latex in Allium cepa test. Biosci J. 2017;33(5):1295–1304. doi: 10.14393/BJ-v33n5a2017-33835. - DOI
    1. Coêlho MDG, Coêlho FAS, Mancilha IM. Probiotic therapy: a promising strategy for the control of canine hookworm. J Parasitol Res. 2013;2013:1–6. - PMC - PubMed

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