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. 2021 Mar 8;7(3):e06248.
doi: 10.1016/j.heliyon.2021.e06248. eCollection 2021 Mar.

Influence of Piper betle L. extract on umbilical cord cells in vitro and potential treating cutaneous wound

Cẩm Hà Chế Thị  1 , Hữu Đạt Nguyễn  1 , Duy Minh Lê Hoàng  1   2
Affiliations

Influence of Piper betle L. extract on umbilical cord cells in vitro and potential treating cutaneous wound

Cẩm Hà Chế Thị et al. Heliyon. .

Abstract

This research aimed to test the effects of Piper betle L. from Vietnam on fibroblasts and UC-derived mesenchymal stem cells (UC-MSCs) from human umbilical cord (UC) on the scratch assay. We tested the extract at different concentrations and then assessed the level of expression of the factors involved in the inflammatory process on fibroblasts including IL-33, VCAM, CD248 by assay real time qPCR. At the concentrations of 0.025 μL/mL and 0.03 μL/mL, the extracts positively affected fibroblast proliferation and UC-MSCs. By contrast, the concentration of 0.058 μL/mL, the extract was toxic to UC-MSCs and fibroblast cell lines, the cells were no longer able to survive. qPCR results show that Piper betle L. extract has the ability to reduce the expression levels of IL-33 (50.8%), VCAM (32.1%), CD248 (46.13%) which trigger inflammatory processes, thereby reducing cellular stress and promoting the process of healing scratches. Our study revealed the proliferation capabilities of Piper betle L. extract from Vietnam In vitro. Hence, Piper betle L. could be recommended as a potential source of wound healing agents.

Keywords: Fibroblast cell; Mesenchymal stem cells; Piper betle L.; The extract.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
GCMS results.
Figure 2
Figure 2
Isolated culture of UC-MSCs and fibroblast. (A) Morphology of UC-MSCs and fibroblast in the primary, first, second and third generation, respectively, (B) Growth curves, (C)colony-forming abilities of UC-MSCs and fibroblast.
Figure 3
Figure 3
Surface markers of UC-MSCs and fibroblast.
Figure 4
Figure 4
Effect of Piper betle L. extracts at different concentrations on UC-MSCs and fibroblast were presented in photos (A,C) and a graph (B,D).
Figure 5
Figure 5
Effect of Piper betle L. extract on scratch wound healing of UC-MSCs. (A,B) Scratch diameter is narrowed, (C) Cell density after 5 days.
Figure 6
Figure 6
Effect of Piper betle L. extract on scratch wound healing of fibroblast. (A,B) Scratch diameter is narrowed, (C) Cell density after 5 days.
Figure 7
Figure 7
Chromosome analysis and expression of the CD 90, α-SMA, CD34, CD45.
Figure 8
Figure 8
Percentage of cells positively stained for samples for expression of CD248, IL-33, VCAM after addition of Piper betle L. extract (∗p < 0.05 (Students t-test)).
See this image and copyright information in PMC

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