Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2021 Mar;8(1):19-23.
doi: 10.1055/s-0041-1723083. Epub 2021 Feb 23.

Evaluation of the Secretor Status of ABO Blood Group Antigens in Saliva using Absorption Inhibition Method

M L Avinash Tejasvi  1 , Jaya Laksmi Bukkya  2 , Pandu Ranga Rao  3 , Harsha Bhayya  4
Affiliations

Evaluation of the Secretor Status of ABO Blood Group Antigens in Saliva using Absorption Inhibition Method

M L Avinash Tejasvi et al. Glob Med Genet. 2021 Mar.

Abstract

Objectives While DNA profiling has become the principal technique for individualization of biological evidences, ABO blood grouping is still a useful test method in the initial stages of crime investigation. Objectives of the study were blood group determination using slide agglutination method, blood group determination from saliva using absorption inhibition method, and comparison of slide agglutination method with that of absorption inhibition method from saliva sample. Materials and Methods A total of 60 subjects were taken randomly with their age ranging from 20 to 60 years. Sixty subjects were divided in to two groups, study group and control group. 5 to 10 mL of unstimulated saliva was collected from 60 patients and Wieners agglutination test was performed to detect the secretor status of blood using absorption inhibition method and compared with that of slide agglutination method Results Out of 60 subjects, 52 subjects showed secretors of antigen in saliva with percentage value of 86.66% and eight subjects were nonsecretors (13.33%). Slightly higher percentage of secretor status was seen in males 84.6 and 88.2% in females. Conclusion Evaluation of secretor status of blood group antigen from saliva using absorption inhibition method can be useful method in identification of medicolegal cases.

Keywords: absorption inhibition method; secretors; slide agglutination method.

PubMed Disclaimer

Conflict of interest statement

Conflict of Interest None declared.

Figures

Fig. 1
Fig. 1
Antisera kit.
Fig. 2
Fig. 2
Centrifuge.
Fig. 3
Fig. 3
Water bath.
Fig. 4
Fig. 4
Micropipettes.
Fig. 5
Fig. 5
Saliva samples after inhibition test.
Fig. 6
Fig. 6
Saliva samples after inhibition test.
See this image and copyright information in PMC

References

    1. Owen R. Karl Landsteiner and the first human marker locus. Genetics. 2000;155(03):995–998. - PMC - PubMed
    1. Mitra R, Mishra N, Rath G P. Blood groups systems. Indian J Anaesth. 2014;58(05):524–528. - PMC - PubMed
    1. Motghare P, Kale L, Bedia A S, Charde S. Efficacy and accuracy of ABO blood group determination from saliva. J Indian Acad Oral Med Radiol. 2011;23:163–167.
    1. Sen M P, Vanishree M, Hunasgi S, Surekha R, Koneru A, Manvikar V. A comparison of absorption inhibition and absorption elution methods for estimation of ABO blood groups in saliva. J Med RadiolPatholSurg. 2015;1:1–4.
    1. Har rington J J, Martin R, Kobilinsky L. Detection of hemagglutinins in dried saliva stains and their potential use in blood typing. J Forensic Sci. 1988;33(03):628–637. - PubMed