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. 2021 Jun;19(6):1089-1091.
doi: 10.1111/pbi.13584. Epub 2021 Apr 1.

Establishing in planta haploid inducer line by edited SiMTL in foxtail millet (Setaria italica)

Zixiang Cheng  1 , Yao Sun  1   2 , Suhua Yang  3 , Hui Zhi  1 , Tao Yin  1 , Xiaojiao Ma  1 , Haoshan Zhang  1 , Xianmin Diao  1 , Yan Guo  2 , Xinhai Li  1 , Chuanyin Wu  1 , Yi Sui  1
Affiliations

Establishing in planta haploid inducer line by edited SiMTL in foxtail millet (Setaria italica)

Zixiang Cheng et al. Plant Biotechnol J. 2021 Jun.
No abstract available

Keywords: SiMTL; CRISPR-Cas9; Foxtail millet; haploid embryo.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Haploid embryo induction in foxtail millet. (a) Genomic structure of SiMTL and targeted sites for the CRISPR‐Cas9 system. PAM is underlined and the guide RNA sequence is highlighted in blue. (b) Five representative T0 plants edited at guide 1 or guide 2 in SiMTL. The predicted consequence at the protein level resulting from each type of editing is given. (c) Seeds setting rate of the same 5 events. Ten T1 plants were grown for each event. For #3 and #5, only homozygous segregants of +1(A)/+1(A) were used for data collection. **Significant difference between simtl and WT at P < 0.01 by two‐tailed t‐test. (d) Representative pollens from a simtl plant, showing normal starch accumulation relative to WT. (e) Verification of haploid plant by flow cytometry analysis. The x‐axis indicates the signal peak for nuclei and y‐axis indicates the number of nuclei. (f) Haploid induction efficiency (HIR%) determined by self‐pollination or crossing. For crossing, a male sterile line 682A was used as the female and was pollinated with the #14 line. (g) Haploid plant with reduced height and smaller organs relative to WT. A representative haploid plant from crossing, morphologically similar to the female parent, is shown in the lower right panel.
See this image and copyright information in PMC

References

    1. Bennetzen, J.L. , Schmutz, J. , Wang, H. , Percifield, R. , Hawkins, J. , Pontaroli, A.C. , Estep, M. et al. (2012) Reference genome sequence of the model plant Setaria. Nat. Biotechnol. 30, 555–561. - PubMed
    1. Gilles, L.M. , Khaled, A. , Laffaire, J.B. , Chaignon, S. , Gendrot, G. , Laplaige, J. , Bergès, H. et al. (2017) Loss of pollen‐specific phospholipase NOT LIKE DAD triggers gynogenesis in maize. Embo J. 36, 707–717. - PMC - PubMed
    1. Jacquier, N.M.A. , Gilles, L.M. , Pyott, D.E. , Martinant, J.P. , Rogowsky, P.M. and Widiez, T. (2020) Puzzling out plant reproduction by haploid induction for innovations in plant breeding. Nat. Plants, 6, 610–619. - PubMed
    1. Jia, G. , Huang, X. , Zhi, H. , Zhao, Y. , Zhao, Q. , Li, W. , Chai, Y. et al. (2013) A haplotype map of genomic variations and genome‐wide association studies of agronomic traits in foxtail millet (Setaria italica). Nat. Genet. 45, 957–961. - PubMed
    1. Jiang, H. , Barbier, H. and Brutnell, T. (2013) Methods for performing crosses in Setaria viridis, a new model system for the grasses. J. Vis. Exp. 80, 50527. - PMC - PubMed

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