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. 2021 Jul;44(7):979-985.
doi: 10.1111/jfd.13356. Epub 2021 Mar 22.

Agreement between the categorization of isolates of Aeromonas salmonicida and Yersinia ruckeri by disc diffusion and MIC tests performed at 22℃

Sandrine Baron  1 , Emeline Larvor  1 , Eric Jouy  1 , Isabelle Kempf  1 , Sophie Le Bouquin  2 , Claire Chauvin  2 , Pierre-Marie Boitard  3 , Matthieu Jamin  3 , Alain Le Breton  4 , Benoit Thuillier  5 , Peter Smith  6
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Agreement between the categorization of isolates of Aeromonas salmonicida and Yersinia ruckeri by disc diffusion and MIC tests performed at 22℃

Sandrine Baron et al. J Fish Dis. 2021 Jul.

Abstract

Standard disc diffusion and MIC test procedure were used to investigate the susceptibility of two hundred and fifty-one isolates collected from infected fish in France to florfenicol, oxolinic acid and tetracycline. The tests were performed at 22 ± 2℃ and for the 177 Yersinia ruckeri they were read after 24-28 hr incubation and for the 74 Aeromonas salmonicida isolates they were read after 44-48 hr. Applying epidemiological cut-off values to the susceptibility data generated in these tests, the isolates were categorized as wild-type or non-wild-type. The agent-specific categories into each isolate were placed on the basis of the data generated by the two methods were in agreement in 98% of the determinations made. It is argued that, with respect to categorising isolates, disc diffusion and MIC methods can be considered as equally valid at this temperature and after both periods of incubation.

Keywords: A. salmonicida; Y. ruckeri; Clinical Laboratory Standard Institute; Minimal Inhibitory Concentration; categorical agreement; disc diffusion.

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