Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Published Erratum
. 2021 Apr 19;49(7):4199-4202.
doi: 10.1093/nar/gkab222.

Corrigendum to article "Quantitative proteomics reveals that long non-coding RNA MALAT1 interacts with DBC1 to regulate p53 acetylation''

Ruibing Chen  1 , Yun Liu  1 , Hao Zhuang  1   2 , Baicai Yang  1 , Kaiwen Hei  1 , Mingming Xiao  1 , Chunyu Hou  1 , Huajun Gao  1 , Xinran Zhang  1 , Chenxi Jia  3   4 , Lingjun Li  3   5 , Yongmei Li  1 , Ning Zhang  1
Affiliations
Published Erratum

Corrigendum to article "Quantitative proteomics reveals that long non-coding RNA MALAT1 interacts with DBC1 to regulate p53 acetylation''

Ruibing Chen et al. Nucleic Acids Res. .
No abstract available

PubMed Disclaimer

Figures

Figure 5.
Figure 5.
MALAT1 regulates cell proliferation and apoptosis in HepG2 cells through binding with DBC1. (A) The growth curve of MALAT1-downregulated cells (left panel) and MALAT1-overexpressed cells (right panel) over 9 days. Cells (2 × 105) were seeded in each well, and the cell numbers were counted every 48 h. Data are from three independent assays. Bar, mean; error bar, SD (*P < 0.05, **P < 0.01, ***P < 0.001, by repeated measures ANOVA). (B) Colony-formation assay of HepG2-MALAT1 and HepG2 cells co-transfected with both MALAT1 and DBC1 or DBC1-N4. Representative images show the results of the colony-formation assay (left panel). Histogram shows the relative colony numbers (right panel). Data are from three independent assays. Bar, mean; error bar, SD (***P < 0.001, by one-way ANOVA). BrdU incorporation analysis of MALAT1-knockdown HepG2 cells (C) and MALAT1-overexpressing cells with or without the overexpression of full-length or N4 fragment of DBC1 respectively (D). Representative images showing alterations in S phase distribution (left panel). Histogram shows the percentage of BrdU staining (right panel). Data are from three independent assays. Bar, mean; error bar, SD (**P < 0.01, ***P < 0.001, by Student's t-test or one-way ANOVA). (E) Annexin V assay for analyzing cell apoptosis in HepG2 cells transfected with shMALAT1 plasmid (left panel) or MALAT1-overexpressing plasmid with or without co-transfection of full-length or N4 fragment of DBC1 respectively (right panel). Data are from three independent assays. Bar, mean; error bar, SD (*P < 0.05, **P < 0.01, ***P < 0.001, by Student's t-test or one-way ANOVA).
Supplemental Figure S2.
Supplemental Figure S2.
Gene ontology analysis of the identified MALAT1-interacting proteins and the validation using RNA immunoprecipitation (RIP). (A) Diagram showing the molecular functions of the identified MALAT1-interacting proteins. (B) Diagram showing the related biological processes of the identified MALAT1-interacting proteins. The annotation information was acquired through the Panther database (http://www.pantherdb.org). Bar graphs present the P values of the enriched protein domains (C) and signaling pathways (D). The protein domain and pathway analyses were performed using DAVID (https://david.ncifcrf.gov/). (E) Agarose gel electrophoresis analysis of the qRT-PCR products of MALAT1, 18S rRNA and NEAT1 in the immunoprecipitates of the selected proteins. IgG was used as negative control.
Supplementary Figure S5.
Supplementary Figure S5.
DBC1 and DBC1-N4 inhibit cell proliferation and promote apoptosis. (A) The overexpression of DBC1 and DBC1-N4 was examined by qRT-PCR. Data represent the means ± SD of triplicate independent analyses (***P < 0.001, by student's t-test). (B) Western blot analysis indicates that DBC1 overexpression increases p53 acetylation. GAPDH expression was used as a control. (C) Growth curve assay using DBC1-overexpressing HepG2 cells. Data represent the means ± SD of triplicate independent analyses (**P < 0.01, by repeated measures ANVOA). (D) BrdU incorporation analysis using DBC1-overexpressing HepG2 cells. Histogram shows the means ± SD from two independent experiments (**P < 0.01, by student's t-test). (E) Colony-formation assay was performed to study the proliferation of HepG2 cells overexpressing full-length DBC1 or DBC1-N4, and their corresponding control cells. Data represent the means ± SD of two independent analyses (**P < 0.01, by one-way ANOVA). (F) Apoptosis analysis of HepG2 cells overexpressing full length DBC1 or DBC1-N4. Apoptotic cells were detected by Annexin V/PI staining assay and examined by flow cytometry. Data represent the means ± SD of triplicate independent analyses (**P < 0.01, ***P < 0.001, by one-way ANOVA).
See this image and copyright information in PMC

Erratum for

Publication types