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. 2021 Dec;64(12):2186-2201.
doi: 10.1007/s11427-020-1878-8. Epub 2021 Mar 16.

Slc20a1b is essential for hematopoietic stem/progenitor cell expansion in zebrafish

Jiakui Chen #  1 , Gaofei Li #  2 , Junwei Lian  2 , Ning Ma  1 , Zhibin Huang  2 , Jianchao Li  2 , Zilong Wen  3 , Wenqing Zhang  4 , Yiyue Zhang  5   6
Affiliations

Slc20a1b is essential for hematopoietic stem/progenitor cell expansion in zebrafish

Jiakui Chen et al. Sci China Life Sci. 2021 Dec.

Abstract

Hematopoietic stem and progenitor cells (HSPCs) are able to self-renew and can give rise to all blood lineages throughout their lifetime, yet the mechanisms regulating HSPC development have yet to be discovered. In this study, we characterized a hematopoiesis defective zebrafish mutant line named smu07, which was obtained from our previous forward genetic screening, and found the HSPC expansion deficiency in the mutant. Positional cloning identified that slc20a1b, which encodes a sodium phosphate cotransporter, contributed to the smu07 blood phenotype. Further analysis demonstrated that mutation of slc20a1b affects HSPC expansion through cell cycle arrest at G2/M phases in a cell-autonomous manner. Our study shows that slc20a1b is a vital regulator for HSPC proliferation in zebrafish early hematopoiesis and provides valuable insights into HSPC development.

Keywords: HSPC; slc20a1b; zebrafish.

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