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. 1988 Jun;85(11):3802-4.
doi: 10.1073/pnas.85.11.3802.

Electron microscopic study of crystals of the Xenopus laevis transcription factor IIIA-5S ribosomal RNA complex

R S Brown  1 , C Ferguson, A Kingswell, F K Winkler, K R Leonard
Affiliations

Electron microscopic study of crystals of the Xenopus laevis transcription factor IIIA-5S ribosomal RNA complex

R S Brown et al. Proc Natl Acad Sci U S A. 1988 Jun.

Abstract

A novel method has been developed to grow crystals of the Xenopus laevis transcription factor IIIA-5S RNA complex directly on grids for examination by electron microscopy. Microcrystals were examined in negative stain and in thin sections to reveal a hexagonal lattice with unit-cell dimensions a = b = 87.1 +/- 4.4 A and c = 143.8 +/- 12.7 A. Optical diffraction patterns from micrographs were obtained about the major crystal axes extending to about 40-A resolution. A packing scheme is proposed for which there are three or six transcription factor IIIA-5S RNA complexes in the unit cell related by 3(1) symmetry along the long cell axis. This would require that the 5S RNA molecules are arranged end-to-end, with the terminal loops of adjacent molecules overlapping.

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