[Evaluation of the efficiency of the adsorption-elution technic for poliovirus 1 on fiber glass filters: application in the virological analysis of 100 ml to 1000 1 of water]

Abstract

The efficiency of the adsorption-elution technique using fiber glass filters to concentrate viruses from water was evaluated to detect poliovirus type 1 in drinking, river, and sewage water. At pH 3.5 and with 5 X 10(-4) M aluminium chloride more than 99% were adsorbed at a 0.25-micron filter. Beef extract (3%), pH 9, eluted 85-95% of the adsorbed viruses and organic flocculation at pH 3.5 permitted to reconcentrate the viruses in 1/20 of the elution volume with a 50-72% efficiency. The overall efficiency of the technique for 100 ml to 1000 l of the different types of water using 10(2) to 10(6) PFU was 38 to 58%.