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Review
. 2021 Mar 23;403(5-6):455-477.
doi: 10.1515/hsz-2020-0377. Print 2022 Apr 26.

Antibody display technologies: selecting the cream of the crop

Bernhard Valldorf  1 , Steffen C Hinz  2 , Giulio Russo  3   4 , Lukas Pekar  5 , Laura Mohr  6 , Janina Klemm  2 , Achim Doerner  5 , Simon Krah  5 , Michael Hust  4 , Stefan Zielonka  5
Affiliations
Free article
Review

Antibody display technologies: selecting the cream of the crop

Bernhard Valldorf et al. Biol Chem. .
Free article

Abstract

Antibody display technologies enable the successful isolation of antigen-specific antibodies with therapeutic potential. The key feature that facilitates the selection of an antibody with prescribed properties is the coupling of the protein variant to its genetic information and is referred to as genotype phenotype coupling. There are several different platform technologies based on prokaryotic organisms as well as strategies employing higher eukaryotes. Among those, phage display is the most established system with more than a dozen of therapeutic antibodies approved for therapy that have been discovered or engineered using this approach. In recent years several other technologies gained a certain level of maturity, most strikingly mammalian display. In this review, we delineate the most important selection systems with respect to antibody generation with an emphasis on recent developments.

Keywords: antibody display; antibody engineering; antibody screening; antibody selection; genotype phenotype coupling.

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