Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2021:2283:175-190.
doi: 10.1007/978-1-0716-1302-3_16.

Evaluating Host Responses to Helicobacter pylori Using ELISA and Western Blot

Ciara Nulty  1 , Gillian Barber  1 , Ewelina Flis  1 , Emma M Creagh  2
Affiliations

Evaluating Host Responses to Helicobacter pylori Using ELISA and Western Blot

Ciara Nulty et al. Methods Mol Biol. 2021.

Abstract

Western blot and enzyme-linked immunosorbent assay (ELISA) are antibody-mediated techniques which are widely used for the detection and characterization of alterations in host protein expression following H. pylori infection . Both techniques are highly specific and sensitive for protein detection, with Western blot detection sensitivity as low as picogram amounts of the protein of interest, while the typical ELISA detection range is 0.01-0.1 ng. Here we provide an experimental example to demonstrate the application of these techniques for the determination of macrophage inflammatory responses following H. pylori infection .

Keywords: ELISA; Inflammatory cytokines; Innate Immune response; Protein expression; Western.

PubMed Disclaimer

References

    1. Weischenfeldt J, Porse B (2008) Bone marrow-derived macrophages (BMM): isolation and applications. CSH Protoc 2008:pdb.prot5080 - PubMed
    1. Semper RP, Mejias-Luque R, Gross C et al (2014) Helicobacter pylori-induced IL-1beta secretion in innate immune cells is regulated by the NLRP3 inflammasome and requires the Cag pathogenicity island. J Immunol 193:3566–3576 - DOI
    1. Kim DJ, Park JH, Franchi L et al (2013) The Cag pathogenicity island and interaction between TLR2/NOD2 and NLRP3 regulate IL-1beta production in Helicobacter pylori-infected dendritic cells. Eur J Immunol 43:2650–2658 - DOI
    1. Koch KN, Hartung ML, Urban S et al (2015) Helicobacter urease-induced activation of the TLR2/NLRP3/IL-18 axis protects against asthma. J Clin Invest 125:3297–3302 - DOI
    1. Gravel P (2009) Protein blotting by the semidry method. In: Walker JM (ed) The protein protocols handbook. Springer Protocols Handbooks. Humana Press, Totowa, NJ

Publication types

LinkOut - more resources