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Observational Study
. 2021 Mar 25;21(1):320.
doi: 10.1186/s12885-021-08045-7.

CD47 expression and CD163+ macrophages correlated with prognosis of pancreatic neuroendocrine tumor

Rami Imam  1 , Qing Chang  1 , Margaret Black  1 , Caroline Yu  1 , Wenqing Cao  2
Affiliations
Observational Study

CD47 expression and CD163+ macrophages correlated with prognosis of pancreatic neuroendocrine tumor

Rami Imam et al. BMC Cancer. .

Abstract

Background: Recent studies have suggested the important roles of CD47 and tumor-associated macrophages in the prognosis and immunotherapy of various human malignancies. However, the clinical significance of CD47 expression and CD163+ TAMs in pancreatic neuroendocrine tumor (PanNET) remains unclear.

Methods: In this study, 47 well-differentiated PanNET resection specimens were collected. CD47 expression and CD163+ macrophages were evaluated using immunohistochemistry and correlated with clinicopathologic properties.

Results: Positive CD47 staining was seen in all PanNETs as well as adjacent normal islets. Compared to normal islets, CD47 overexpressed in PanNETs (p = 0.0015). In the cohort, lymph node metastasis (LNM), lymphovascular invasion (LVI), and perineural invasion (PNI) were found in 36.2, 59.6, and 48.9% of the cases, respectively. Interestingly, PanNETs with LNM, LVI, or PNI had significantly lower H-score of CD47 than those without LNM (p = 0.035), LVI (p = 0.0005), or PNI (p = 0.0035). PanNETs in patients with disease progression (recurrence/death) also showed a significantly lower expression of CD47 than those without progression (p = 0.022). In contrast, CD163+ macrophage counts were significantly higher in cases with LNM, LVI, and PNI.

Conclusions: Our data suggest relative low CD47 expression and high CD163+ TAMs may act as indicators for poor prognosis of PanNETs.

Keywords: M2 macrophage; Microenvironment; Pancreatic tumor; Prognosis; Targeting CD47 therapy.

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Conflict of interest statement

The authors declare that they have no competing interests.

Figures

Fig. 1
Fig. 1
Characteristics of CD47 in human normal pancreas and PanNETs detected by IHC. a. CD47 expression in normal pancreatic tissue (X100). b. representative image of CD47 expression in PanNET and adjacent normal pancreatic tissue (X200). c. CD47 expression on cell membrane and in cytoplasm at high power view (X400). d. H-score of CD47 in normal islets and PanNETs. e, f and g showed the intensity of CD47 staining in PanNETs (X200). h. CD163+ TAMs in PanNETs (X400). * Significant difference, p < 0.05 with unpaired t test
Fig. 2
Fig. 2
Association of CD47 expression with PanNET prognosis. a, b and c showed the associations of CD47 H-score with LNM, LVI and PNI. d. CD47 expression related to recurrence/death
Fig. 3
Fig. 3
CD163+ TAMs associated with PanNET prognosis. a, b, and c displayed the associations of CD163+ macrophages with LNM, LVI and PNI. d. association of CD163+ macrophage counts with recurrence/death
See this image and copyright information in PMC

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