Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2021 Mar 23;5(4):e550.
doi: 10.1097/HS9.0000000000000550. eCollection 2021 Apr.

A Major Subset of Mutated CLL Expresses Affinity-selected and Functionally Proficient Rheumatoid Factors

Jerry Janssen  1   2 , Naomi Donner  1   2 , Zhen Li  3 , Thera A M Wormhoudt  1   2 , Koen Wagner  4 , Jeroen E J Guikema  1   2 , C Ellen van der Schoot  5 , Arnon P Kater  2   6   7 , Ten Feizi  3 , Richard J Bende  1   2 , Carel J M van Noesel  1   2
Affiliations

A Major Subset of Mutated CLL Expresses Affinity-selected and Functionally Proficient Rheumatoid Factors

Jerry Janssen et al. Hemasphere. .
No abstract available

PubMed Disclaimer

Figures

Figure 1.
Figure 1.
The BCRs of CLLs with RF reactivity are affinity-selected and mediate tumor cell proliferation in response to ligation by human IgG. (A), Six out of 152 CLL IgM specifically bind IgG in ELISA. Controls are IgM of CLL57, which is polyreactive, and IgM (V3-7Sh-1) of CLL82, which is specific for β-(1,6)-glucan. (B), IgG-binding affinities of IgMs derived from the CLLs, including CLL115 and CLL189, and their IGHV-germline-reverted variants (CLL115-VH-GL, CLL189-VH-GL) as measured by surface plasmon resonance (IBIS MX96, IBIS Technologies, The Netherlands). (C), Binding characteristics of IgMs from CLL115, CLL115-GL, CLL189, and CLL189-GL with coated IgG in ELISA. (D), Primary cells of CLL189, CLL243, and CLL253 proliferate upon interaction with their cognate antigen (IgG) or with anti-IgM monoclonal antibodies. Fresh CLL cells were labelled with CFSE, cultured for 8 days and analyzed by flow cytometry. BCRs = B-cell receptors; CFSE = carboxyfluorescein succinimidyl ester; CLLs = chronic lymphocytic leukemias; IGHV = immunoglobulin heavy chain variable region; RF = rheumatoid factor.
Figure 2.
Figure 2.
The configuration of IGHV and IGKV of RF CLL. (A), IGHV and IGKV rearrangements and VH-CDR3 of 6 RF CLLs. (B), Alignment of amino acid sequences of the IGHV1-69-, IGHV3-30-, and IGKV3-20-expressing CLLs. Indicated in red a S36R shared between 4 CLL. (C), Comparison of the VH-CDR3 amino acid sequences expressed by CLL226, a salivary gland MALT lymphoma, and an ocular adnexal MALT lymphoma, respectively. VH-CDR3 homology is calculated as the fraction of identical/similar amino acids. Identical amino acids are depicted in red and similar amino acids are depicted in blue. CLLs = chronic lymphocytic leukemias; IGHV = immunoglobulin heavy chain variable region; IGKV = immunoglobulin kappa chain variable region; RF = rheumatoid factor.
See this image and copyright information in PMC

References

    1. Agathangelidis A, Darzentas N, Hadzidimitriou A, et al. . Stereotyped B-cell receptors in one-third of chronic lymphocytic leukemia: a molecular classification with implications for targeted therapies. Blood. 2012; 119:4467–4475. - PMC - PubMed
    1. Kostareli E, Gounari M, Janus A, et al. . Antigen receptor stereotypy across B-cell lymphoproliferations: the case of IGHV4-59/IGKV3-20 receptors with rheumatoid factor activity. Leukemia. 2012; 26:1127–1131. - PubMed
    1. Hoogeboom R, Wormhoudt TA, Schipperus MR, et al. . A novel chronic lymphocytic leukemia subset expressing mutated IGHV3-7-encoded rheumatoid factor B-cell receptors that are functionally proficient. Leukemia. 2013; 27:738–740. - PubMed
    1. Martin T, Duffy SF, Carson DA, et al. . Evidence for somatic selection of natural autoantibodies. J Exp Med. 1992; 175:983–991. - PMC - PubMed
    1. Pritsch O, Magnac C, Dumas G, et al. . V gene usage by seven hybrids derived from CD5+ B-cell chronic lymphocytic leukemia and displaying autoantibody activity. Blood. 1993; 82:3103–3112. - PubMed

LinkOut - more resources