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. 2021 Apr;95(4):1251-1266.
doi: 10.1007/s00204-021-02998-1. Epub 2021 Mar 29.

Systemic effects and impact on the gut microbiota upon subacute oral exposure to silver acetate in rats

Dominique Lison  1 , Jérôme Ambroise  2 , Riccardo Leinardi  3 , Saloua Ibouraadaten  3 , Yousof Yakoub  3 , Gladys Deumer  4 , Vincent Haufroid  3   4 , Adrien Paquot  5 , Giulio G Muccioli  5 , Sybille van den Brûle  3
Affiliations

Systemic effects and impact on the gut microbiota upon subacute oral exposure to silver acetate in rats

Dominique Lison et al. Arch Toxicol. 2021 Apr.

Abstract

Context: The addition of silver (Ag) to food items, and its migration from food packaging and appliances results in a dietary exposure in humans, estimated to 70-90 µg Ag/day. In view of the well-known bactericidal activity of Ag ions, concerns arise about a possible impact of dietary Ag on the gut microbiota (GM), which is a master determinant of human health and diseases. Repeated oral administration of Ag acetate (AgAc) can also cause systemic toxicity in rats with reported NOAELs of 4 mg AgAc/b.w./d for impaired fertility and 0.4 mg AgAc/b.w./d for developmental toxicity.

Objective: The objective of this study was to investigate whether oral exposure to AgAc can induce GM alterations at doses causing reproductive toxicity in rats.

Methods: Male and female Wistar rats were exposed during 10 weeks to AgAc incorporated into food (0, 0.4, 4 or 40 mg/kg b.w./d), and we analyzed the composition of the GM (α- and β-diversity). We documented bacterial function by measuring short-chain fatty acid (SCFA) production in cecal content. Ferroxidase activity, a biomarker of systemic Ag toxicity, was measured in serum.

Results and conclusions: From 4 mg/kg b.w./d onwards, we recorded systemic toxicity, as indicated by the reduction of serum ferroxidase activity, as well as serum Cu and Se concentrations. This systemic toxic response to AgAc might contribute to explain reprotoxic manifestations. We observed a dose-dependent modification of the GM composition in male rats exposed to AgAc. No impact of AgAc exposure on the production of bacterial SCFA was recorded. The limited GM changes recorded in this study do not appear related to a reprotoxicity outcome.

Keywords: Ceruloplasmin; Dysbiosis; Ferroxidase; Reprotoxicity; Silver.

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References

    1. Azad MB, Konya T, Maughan H et al (2013) Infant gut microbiota and the hygiene hypothesis of allergic disease: impact of household pets and siblings on microbiota composition and diversity. Allergy Asthma Clin Immunol 9(1):15. https://doi.org/10.1186/1710-1492-9-15 - DOI - PubMed - PMC
    1. Barras F, Aussel L, Ezraty B (2018) Silver and antibiotic, new facts to an old story. Antibiotics (Basel). https://doi.org/10.3390/antibiotics7030079 - DOI
    1. Benjamini Y, Hochberg Y (1995) Controlling the false discovery rate: a practical and powerful approach to multiple testing. J R Stat Soc B (Methodol) 57(1):289–300. https://doi.org/10.1111/j.2517-6161.1995.tb02031.x - DOI
    1. Bibbo S, Abbondio M, Sau R et al (2020) Fecal microbiota signatures in celiac disease patients with poly-autoimmunity. Front Cell Infect Microbiol 10:349. https://doi.org/10.3389/fcimb.2020.00349 - DOI - PubMed - PMC
    1. Bokulich NA, Subramanian S, Faith JJ et al (2013) Quality-filtering vastly improves diversity estimates from Illumina amplicon sequencing. Nat Methods 10(1):57–59. https://doi.org/10.1038/nmeth.2276 - DOI - PubMed - PMC

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