Surgical ventricular restoration for rabbit dilated cardiomyopathy model: Preliminary study

Abstract

Dilated cardiomyopathy (DCM) is a common cardiovascular disease in dogs. It is primarily treated with beta-blockers. The aim of this preliminary study was to evaluate the efficacy of surgical ventricular restoration on daunorubicin-induced DCM in rabbits. The DCM model was generated in six rabbits by intravenous administration of daunorubicin (4 mg/kg/week) for 6 weeks. Echocardiography was performed before and after left ventricular (LV) reconstruction surgery to evaluate the LV function. The surgery was performed using non-absorbent prosthetic sheets and 5-0 polypropylene sutures, between the left anterior descending branch and the circumflex branch of the left coronary artery. We measured LV function 3 weeks postoperatively and collected the hearts for histopathological examination. We observed significant improvements in the LV internal dimension volume at both end-diastole and end-systole compared to the preoperative values. Fraction shortening showed a substantial improvement from 23.8 ± 1.4% to 30.9 ± 1.3%. In addition, the ejection fraction increased from 35.3% to 51.9%. On histological examination, the left ventricular wall was markedly thin. Furthermore, we observed fibrosis between the myocardial cells around the sutured site. The LV volume and LV functions significantly improved postoperatively. However, further investigation is required to determine the long-term effects of surgical ventricular restoration.

Keywords: Daunorubicin; Dilated cardiomyopathy; Left ventricular reconstruction; Rabbit model; Surgery.