Enhancing methanogenic fermentation of waste activated sludge via isoelectric-point pretreatment: Insights from interfacial thermodynamics, electron transfer and microbial community

Abstract

The usefulness of waste activated sludge (WAS) as an energy source is limited by the poor generation efficiency of methane from WAS, which is mainly due to the complex nature of sludge and low abundance of functional microbes. In this study, the interfacial thermodynamics, electron transfer and microbial community of sludge were investigated to reveal the enhancing effects of isoelectric-point (pI) pretreatment on the efficiency of methane generation from WAS. Experimentally, after pI pretreatment, the methane production potential, maximum methane production rate and maximum methane proportion in the biogas increased by 122.2%, 154.4% and 17.4%, respectively, indicating that pI pretreatment enhanced the generation efficiency of methane. Analyses of changes in the solid-liquid interfacial non-covalent interaction energy, electron transfer capacity (ETC) and reductive peak potential values of sludge samples with and without pI pretreatment during a 170-day methanogenic fermentation period revealed that pI pretreatment enhanced the self-driven solid-liquid interfacial hydrophobic attractions of sludge, increased the abiotic driving forces of interfacial enzymatic reactions, promoted the electron transfer efficiency and lowered the barrier of the reduction reaction. It was thus hypothesised that these changes would be responsible for increasing methane production, which was confirmed by the correlation analyses between the interfacial free energy (IFE) and ETC versus daily methane production. Moreover, statistical analyses of the differences between the microbial communities of sludge samples with and without pI pretreatment during fermentation demonstrated that pI pretreatment significantly (P < 0.05) improved the relative abundances of the main functional microbes with respect to hydrolysis, acidification and methanation. A further investigation of the relationships of IFE and ETC with the relative abundances of the main genera of methanogens indicated that the hydrophobic attraction of sludge surface and a high ETC are conducive to the enrichment of hydrogenotrophic methanogens (+29.9%). These findings are expected to provide a conceptual framework for developing second-generation pretreatment methods and provide a methodological reference for revealing the details of the 'black-box' anaerobic digestion process.

Keywords: Anaerobic digestion; Bio-energy; Biodegradability; Electrochemical characteristics; Hydrophobic interaction; Sewage sludge.