Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2021 Mar 31;19(1):50.
doi: 10.1186/s43141-021-00145-y.

Characterization and optimization of extracellular enzymes production by Aspergillus niger strains isolated from date by-products

Reda Bellaouchi  1 , Houssam Abouloifa  2 , Yahya Rokni  2 , Amina Hasnaoui  2 , Nabil Ghabbour  2 , Abdelkader Hakkou  2 , Abdelmajid Bechchari  3 , Abdeslam Asehraou  2
Affiliations

Characterization and optimization of extracellular enzymes production by Aspergillus niger strains isolated from date by-products

Reda Bellaouchi et al. J Genet Eng Biotechnol. .

Abstract

Background: This work aims to study the optimal conditions of the fermentation culture medium used for the production of extracellular enzymes (amylase, cellulase, lipase, and protease) from previously isolated Aspergillus niger strains in date by-products.

Results: The five most powerful isolates selected based on the zone of degradation formed on Petri plates by the substrate were subjected to the quantitative evaluation of their enzymatic production. All five strains showed almost similar API-ZYM profiles, with minor variations observed at the level of some specific enzyme expression. The production of cellulase and amylase was depending on pH and incubation temperatures. ASP2 strain demonstrated the high production rate of amylase (at pH 5 and 30 °C) and cellulase (at pH 6 and 30 °C) for 96 h of incubation.

Conclusion: The A. niger showed the ability to produce several extracellular enzymes and can be used in the valorization of different agroindustrial residues.

Keywords: Aspergillus niger; Date by-products; Enzymes; Extracellular.

PubMed Disclaimer

Conflict of interest statement

The authors declare that they have no competing interests.

Figures

Fig. 1
Fig. 1
Cellulase activity of Aspergillus niger in plate agar medium
Fig. 2
Fig. 2
Effect of initial pH on production of amylase and cellulase enzymes by A. niger ASP2. Values are mean ± standard error of triplicates. a–f Means in same column of each parameter with different lower case letters differed significantly (p < 0.05). A, B Values in the enzymatic activity (cellulase and amylase) for each parameter with different lower case letters differed significantly (p < 0.05)
Fig. 3
Fig. 3
Effect of incubation temperature on production of amylase and cellulase enzymes A. niger ASP2. Values are mean ± standard error of triplicates. a–f Means in same column of each parameter with different lower case letters differed significantly (p < 0.05). A, B Values in the enzymatic activity (cellulase and amylase) for each parameter with different lower case letters differed significantly (p < 0.05)
Fig. 4
Fig. 4
Effect of incubation time on production of amylase and cellulase enzymes A. niger ASP2
See this image and copyright information in PMC

References

    1. Berka RM, Dunn-Coleman N, Ward M. Industrial enzymes from Aspergillus species. Biotechnol. 1992;23:155. - PubMed
    1. Pandey A, Selvakumar P, Soccol CR, Poonam Nigam (1999) Solid-state fermentation for the production of industrial enzymes. Curr Sci:149–162
    1. Coutinho PM, Andersen MR, Kolenova K, vanKuyk PA, Benoit I, Gruben BS, Trejo-Aguilar B, Visser H, van Solingen P, Pakula T. Post-genomic insights into the plant polysaccharide degradation potential of Aspergillus nidulans and comparison to Aspergillus niger and Aspergillus oryzae. Fungal Genet Biol. 2009;46(1):S161–S169. doi: 10.1016/j.fgb.2008.07.020. - DOI - PubMed
    1. de Vries RP, Visser J. Aspergillus enzymes involved in degradation of plant cell wall polysaccharides. Microbiol Mol Biol Rev. 2001;65(4):497–522. doi: 10.1128/MMBR.65.4.497-522.2001. - DOI - PMC - PubMed
    1. Bellaouchi R, Ghomari I, Hasnaoui A, Hakkou A, Bechchari A, Chihib NE, Asehraou A. Physico-chemical and microbial properties of undervalued dates and processed dates by-products in Morocco. Int Food Re J. 2017;24:963–969.

LinkOut - more resources