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. 1977 Nov;34(5):518-22.
doi: 10.1128/aem.34.5.518-522.1977.

Double-antibody solid-phase enzyme immunoassay for the detection of staphylococcal enterotoxin A

G C Saunders, M L Bartlett

Double-antibody solid-phase enzyme immunoassay for the detection of staphylococcal enterotoxin A

G C Saunders et al. Appl Environ Microbiol. 1977 Nov.

Abstract

A simple double-antibody enzyme immunoassay that uses a microtechnique was developed for detecting staphylococcal enterotoxin A in food products. Sample preparation can be completed in less than 15 min. Assay sensitivity ranges from 0.4 ng (20-h test time) to 3.2 ng (1- to 3-h test time) of toxin per ml of prepared sample. Separation and detection of enterotoxin from spiked food products ranged between 72 and 98% of the amount added.

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References

    1. J Clin Pathol. 1976 Oct;29(10):873-9 - PubMed
    1. Appl Microbiol. 1968 Jul;16(7):1019-23 - PubMed
    1. J Histochem Cytochem. 1974 Dec;22(12):1084-91 - PubMed
    1. J Immunol. 1972 Mar;108(3):852-6 - PubMed
    1. Appl Microbiol. 1973 Sep;26(3):309-13 - PubMed

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