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. 2021 Apr 1;16(4):e0249684.
doi: 10.1371/journal.pone.0249684. eCollection 2021.

Expression of Concern: Sox9 Potentiates BMP2-Induced Chondrogenic Differentiation and Inhibits BMP2-Induced Osteogenic Differentiation

PLOS ONE Editors

Expression of Concern: Sox9 Potentiates BMP2-Induced Chondrogenic Differentiation and Inhibits BMP2-Induced Osteogenic Differentiation

PLOS ONE Editors. PLoS One. .
No abstract available

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Figures

Fig 2
Fig 2. Sox9 potentiates BMP2-induced glycosaminoglycans synthesis in MSCs in micromass cultures.
(A): Real-time PCR for the expression of chondrogenic differentiation marker gene ACAN were conducted on day 7 and day 14 after infection of AdGFP, AdBMP2, and/or AdSox9, using GAPDH as a house keeping gene. (B–C): Alcian blue staining for sulfated glycosaminoglycans in micromass cultures of C3H10T1/2 cells on day 7 and day 14 after transduction of indicated recombinant adenoviruses, gross observation (B) and microscope examination (C, 40X) are shown. (D): Alcian blue staining quantifying: cells were extracted with 6 M guanidine hydrochloride, Optical density of the extracted dye was measured at 630 nm. The results were expressed as mean±SD of triplicate experiments, *P<0.05, #P<0.01.
Fig 5
Fig 5. Sox9 inhibits BMP2-induced osteogenic markers expression in MSCs in vitro.
(A): Real-time PCR for the expression of early osteogenic differentiation gene Runx2 (Aa), late osteogenic gene osteocalcin (OC) and osteopontin (OPN) (b, c) were conducted at indicated time points after infection with AdGFP, AdBMP2, and/or AdSox9, using GAPDH as a house keeping gene. (B): Western blot for the expression of Runx2 (a), OPN (b) and OC (c) were conducted at indicated time points after transduction of indicated recombinant adenoviruses, respectively. (C) Relative protein expression was analyzed by quantity one software using β-actin as controls respectively. The results are expressed as mean±SD of triplicate experiments, *P<0.05, #P<0.01.
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Expression of concern for

References

    1. Liao J, Hu N, Zhou N, Lin L, Zhao C, Yi S, et al.. (2014) Sox9 Potentiates BMP2-Induced Chondrogenic Differentiation and Inhibits BMP2-Induced Osteogenic Differentiation. PLoS ONE 9(2): e89025. 10.1371/journal.pone.0089025 - DOI - PMC - PubMed

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