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. 2021 Mar 17;6(3):948-950.
doi: 10.1080/23802359.2021.1889413.

The complete chloroplast genome of Ocimum gratissimum from India - a medicinal plant in the Lamiaceae

Raju Balaji  1 , Kumar Ravichandiran  1 , Tanuja  1 , Madasamy Parani  1
Affiliations

The complete chloroplast genome of Ocimum gratissimum from India - a medicinal plant in the Lamiaceae

Raju Balaji et al. Mitochondrial DNA B Resour. .

Abstract

Ocimum gratissimum L. is an important medicinal species with several therapeutic applications. It is used in traditional medicine as a single drug and in formulations. We generated the complete chloroplast genome sequence of O. gratissimum by using Illumina paired-end sequencing data. The O. gratissimum chloroplast genome is 152,469 bp in length, containing a large single copy (LSC) region of 83,614 bp and a small single copy region (SSC) of 17,607 bp, separated by a pair of inverted repeats (IRs) of 25,624 bp. The genome contains 138 unique genes, including 85 protein-coding, 45 tRNA, and eight rRNA genes. Among them, six genes have one intron each, and two genes contain two introns. The overall GC content of the chloroplast genome is 37.8%, while the corresponding values of LSC, SSC, and IR regions are 35.6%, 31.7%, and 43.2%, respectively. Phylogenetic analysis with the complete chloroplast genomes of other related species revealed that O. gratissimum is fully resolved in a clade with other Ocimum species classified to the family Lamiaceae.

Keywords: Complete chloroplast genome; Lamiaceae; Ocimum gratissimum; Tulsi.

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Conflict of interest statement

No potential conflict of interest was reported by the author(s).

Figures

Figure 1.
Figure 1.
Neighbor-joining (NJ) tree based on the whole chloroplast genome sequences of 21 species including Nicotiana tabacum L. and Arabidopsis thaliana L. as outgroups. The complete chloroplast genome sequences were aligned using MAFFT online version (https://mafft.cbrc.jp/alignment/server/) and subjected to generating NJ phylogenetic tree by MEGA v7.0 (Kumar et al. 2016). The bootstrap support values (>50%) from 1000 replicates are indicated in the nodes.
See this image and copyright information in PMC

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