Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2021 Jun;44(6):1908-1920.
doi: 10.1111/pce.14059. Epub 2021 Apr 13.

Medicago truncatula Yellow Stripe-Like7 encodes a peptide transporter participating in symbiotic nitrogen fixation

Rosario Castro-Rodríguez  1 , Viviana Escudero  1 , María Reguera  1 , Patricia Gil-Díez  1 , Julia Quintana  2 , Rosa Isabel Prieto  1 , Rakesh K Kumar  3 , Ella Brear  4 , Louis Grillet  5 , Jiangqi Wen  6 , Kirankumar S Mysore  6 , Elsbeth L Walker  3 , Penelope M C Smith  4 , Juan Imperial  7 , Manuel González-Guerrero  1   8
Affiliations
Free article

Medicago truncatula Yellow Stripe-Like7 encodes a peptide transporter participating in symbiotic nitrogen fixation

Rosario Castro-Rodríguez et al. Plant Cell Environ. 2021 Jun.
Free article

Abstract

Yellow Stripe-Like (YSL) proteins are a family of plant transporters that are typically involved in transition metal homeostasis. Three of the four YSL clades (I, II and IV) transport metals complexed with the non-proteinogenic amino acid nicotianamine or its derivatives. No such capability has been shown for any member of clade III, but the link between these YSLs and metal homeostasis could be masked by functional redundancy. We studied the role of the clade III YSL protein MtSYL7 in Medicago truncatula nodules. MtYSL7, which encodes a plasma membrane-bound protein, is mainly expressed in the pericycle and cortex cells of the root nodules. Yeast complementation assays revealed that MtSYL7 can transport short peptides. M. truncatula transposon insertion mutants with decreased expression of MtYSL7 had lower nitrogen fixation rates and showed reduced plant growth whether grown in symbiosis with rhizobia or not. YSL7 mutants accumulated more copper and iron in the nodules, which is likely to result from the increased expression of iron uptake and delivery genes in roots. Taken together, these data suggest that MtYSL7 plays an important role in the transition metal homeostasis of nodules and symbiotic nitrogen fixation.

Keywords: Medicago truncatula; YSL; peptide transport; symbiotic nitrogen fixation.

PubMed Disclaimer

References

REFERENCES

    1. Andaluz, S., Rodríguez-Celma, J., Abadía, A., Abadía, J., & López-Millán, A. F. (2009). Time course induction of several key enzymes in Medicago truncatula roots in response to Fe deficiency. Plant Physiology and Biochemistry, 47, 1082-1088.
    1. Aoyama, T., Kobayashi, T., Takahashi, M., Nagasaka, S., Usuda, K., Kakei, Y., … Nishizawa, N. K. (2009). OsYSL18 is a rice iron(III)-deoxymugineic acid transporter specifically expressed in reproductive organs and phloem of lamina joints. Plant Molecular Biology, 70, 681-692.
    1. Askwith, C., Eide, D., Van, H. A., Bernard, P. S., Li, L., Davis-Kaplan, S., … Kaplan, J. (1994). The FET3 gene of S. cerevisiae encodes a multicopper oxidase required for ferrous iron uptake. Cell, 76, 403-410.
    1. Beadle, G. W. (1929). Yellow stripe-a factor for chlorophyll deficiency in maize located in the Pr pr chromosome. The American Naturalist, 63, 189-192.
    1. Boisson-Dernier, A., Chabaud, M., Garcia, F., Bécard, G., Rosenberg, C., & Barker, D. G. (2001). Agrobacterium rhizogenes-transformed roots of Medicago truncatula for the study of nitrogen-fixing and endomycorrhizal symbiotic associations. Molecular Plant-Microbe Interactions, 14, 695-700.

Publication types

LinkOut - more resources