Experimental and Theoretical Approaches to Describing Interactions in Natural Cell Membranes Occurring as a Result of Fatal Alcohol Poisoning

Abstract

We propose herein a theoretical model describing the effect of fatal ethanol poisoning on the equilibria between cell membranes and the surrounding ions. Using this model, we determined the parameters characterizing the interaction between the electrolyte solution's ions and the functional groups on the blood cells' surface. Via the application of mathematical equations, we calculated the total surface concentrations of the acidic and basic groups, c_A and c_B, and their association constants with solution ions, K_AH and K_BOH. Using the determined parameters and mathematical equations' values, we calculated the theoretical surface charge density values. We verified the proposed model by comparing these values with experimental data, which were selected based on measurements of the electrophoretic mobility of erythrocyte and thrombocyte membranes. Compatibility of the experimental and theoretical surface charge density values was observed in the range of pH 2-8, while deviations were observed at higher pH values.

Keywords: acid–base equilibria; erythrocytes; fatal ethyl alcohol poisoning; microelectrophoresis; surface charge density; thrombocytes.

Figure 1

The experimental surface charge density of erythrocytes vs. pH of the electrolyte solution ●—control, ■—fatal ethyl alcohol poisoning.

Figure 2

The experimental surface charge density of thrombocytes vs. pH of the electrolyte solution: ●—control, ■—fatal ethyl alcohol poisoning.

Figure 3

A comparison of experimental and theoretical surface charge density values of erythrocytes vs. pH of the electrolyte solution: ● control, ■ fatal ethyl alcohol poisoning. Points represent the experimental values and curves represent theoretical values.

Figure 4

A comparison of experimental and theoretical surface charge density values of thrombocytes vs. pH of the electrolyte solution: ● control, ■ fatal ethyl alcohol poisoning. Points represent the experimental values and curves represent theoretical values.