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. 2021 Mar 9;12(3):290.
doi: 10.3390/mi12030290.

Development of Rapid and High-Precision Colorimetric Device for Organophosphorus Pesticide Detection Based on Microfluidic Mixer Chip

Jiaqing Xie  1 , Haoran Pang  1 , Ruqian Sun  1 , Tao Wang  2 , Xiaoyu Meng  1 , Zhikang Zhou  1
Affiliations

Development of Rapid and High-Precision Colorimetric Device for Organophosphorus Pesticide Detection Based on Microfluidic Mixer Chip

Jiaqing Xie et al. Micromachines (Basel). .

Abstract

The excessive pesticide residues in cereals, fruit and vegetables is a big threat to human health, and it is necessary to develop a portable, low-cost and high-precision pesticide residue detection scheme to replace the large-scale laboratory testing equipment for rapid detection of pesticide residues. In this study, a colorimetric device for rapid detection of organophosphorus pesticide residues with high precision based on a microfluidic mixer chip was proposed. The microchannel structure with high mixing efficiency was determined by fluid dynamics simulation, while the corresponding microfluidic mixer chip was designed. The microfluidic mixer chip was prepared by a self-developed liquid crystal display (LCD) mask photo-curing machine. The influence of printing parameters on the accuracy of the prepared chip was investigated. The light source with the optimal wavelength of the device was determined by absorption spectrum measurement, and the relationship between the liquid reservoir depth and detection limit was studied by experiments. The correspondence between pesticide concentration and induced voltage was derived. The minimum detection concentration of the device could reach 0.045 mg·L-1 and the average detection time was reduced to 60 s. The results provide a theoretical and experimental basis for portable and high-precision detection of pesticide residues.

Keywords: LCD mask photo-curing; colorimetric device; microfluidics; organophosphorus pesticide residues.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
The principle of color reaction.
Figure 2
Figure 2
Schematic diagram of self-developed liquid crystal display (LCD) mask photo-curing machine.
Figure 3
Figure 3
Schematic diagram of colorimetric device (a), colorimetric device after assembly (b), the absorption spectrogram of the narrow band filter (c), and self-developed mobile client (d).
Figure 4
Figure 4
The designed two types of microstructures with curved channel (a), curved width variation channel (b).
Figure 5
Figure 5
The simulation result of mixing index of two kinds of channels (a) and the simulated concentration distribution at the speed of 50 μL·min−1 (b).
Figure 6
Figure 6
The diagram of the designed microfluidic mixer chip.
Figure 7
Figure 7
Relationship between ultraviolet (UV) exposure time and surface roughness.
Figure 8
Figure 8
The influence of single layer curing thickness on channel size (a), and the cross-section shape of cured microchannel (b).
Figure 9
Figure 9
The microfluidic mixer chip fabricated by liquid crystal display (LCD) mask UV curing method: (a) overall structure and (b) scanning electron microscope (SEM) photograph of microchannel structure.
Figure 10
Figure 10
Absorption spectrum measurement results for 0, 1 and 2 mg∙L−1 concentrations.
Figure 11
Figure 11
Influence of liquid reservoir depth on voltage difference.
Figure 12
Figure 12
Relationship between liquid reservoir depth and detection limit.
Figure 13
Figure 13
Relationship between induced voltage and pesticide concentration.
Figure 14
Figure 14
Color change of mixed liquid with time.
See this image and copyright information in PMC

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