Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1988 May 25;16(10):4525-38.
doi: 10.1093/nar/16.10.4525.

Protein-DNA crosslinking in reconstituted nucleohistone, nuclei and whole cells by picosecond UV laser irradiation

D Angelov  1 , Stefanovsky VYu, S I Dimitrov, V R Russanova, E Keskinova, I G Pashev
Affiliations
Free PMC article

Protein-DNA crosslinking in reconstituted nucleohistone, nuclei and whole cells by picosecond UV laser irradiation

D Angelov et al. Nucleic Acids Res. .
Free PMC article

Abstract

A picosecond UV laser was used to cross-link proteins to DNA in nuclei, whole cells and reconstituted nucleohistone. Irradiation of the nucleohistone resulted in crosslinking 15-20% of bound histones to DNA in a very short time (one or several picosecond pulses), the efficiency of crosslinking to single stranded DNA being higher than to double stranded DNA. All histones as well as high mobility group 1 proteins were identified in the covalently linked protein-DNA complexes upon irradiation of isolated nuclei and whole cells. A method is suggested for isolation of crosslinked material from cells and nuclei in amounts sufficient for further analysis. Experiments with reconstituted nucleohistones showed that upon irradiation at a constant dose the efficiency of crosslinking depended on the intensity of the light, thus suggesting a two-quantum process is involved in the reaction.

PubMed Disclaimer

References

    1. Photochem Photobiol. 1970 May;11(5):349-59 - PubMed
    1. Eur J Biochem. 1987 Sep 1;167(2):321-6 - PubMed
    1. Biochem Biophys Res Commun. 1970;39(6):1011-6 - PubMed
    1. Biochim Biophys Acta. 1972 Nov 9;281(4):522-34 - PubMed
    1. Biochemistry. 1974 Jan 29;13(3):485-93 - PubMed

Publication types