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. 2021 Mar 30;26(7):1932.
doi: 10.3390/molecules26071932.

Insight into Gentisic Acid Antidiabetic Potential Using In Vitro and In Silico Approaches

Hamza Mechchate  1 , Imane Es-Safi  1 , Omkulthom Mohamed Al Kamaly  2 , Dalila Bousta  1
Affiliations

Insight into Gentisic Acid Antidiabetic Potential Using In Vitro and In Silico Approaches

Hamza Mechchate et al. Molecules. .

Abstract

Numerous scientific studies have confirmed the beneficial therapeutic effects of phenolic acids. Among them gentisic acid (GA), a phenolic acid extensively found in many fruit and vegetables has been associated with an enormous confirmed health benefit. The present study aims to evaluate the antidiabetic potential of gentisic acid and highlight its mechanisms of action following in silico and in vitro approaches. The in silico study was intended to predict the interaction of GA with eight different receptors highly involved in the management and complications of diabetes (dipeptidyl-peptidase 4 (DPP4), protein tyrosine phosphatase 1B (PTP1B), free fatty acid receptor 1 (FFAR1), aldose reductase (AldR), glycogen phosphorylase (GP), α-amylase, peroxisome proliferator-activated receptor gamma (PPAR-γ) and α-glucosidase), while the in vitro study studied the potential inhibitory effect of GA against α-amylase and α-glucosidase. The results indicate that GA interacted moderately with most of the receptors and had a moderate inhibitory activity during the in vitro tests. The study therefore encourages further in vivo studies to confirm the given results.

Keywords: AldR; DPP4; FFAR1; GP; PPAR-γ; PTP1B; gentisic acid; in silico; in vitro; molecular docking; α-amylase; α-glucosidase.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Two-dimensional (2D) scheme of the gentisic acid (GA) interactions with protein tyrosine phosphatase 1B (PTP1B) receptor.
Figure 2
Figure 2
Two-dimensional scheme of the GA interactions with dipeptidyl-peptidase 4 (DPP4) receptor.
Figure 3
Figure 3
Two-dimensional scheme of the GA interactions with α-amylase receptor.
Figure 4
Figure 4
Two-dimensional scheme of the GA interactions with α-glucosidase receptor.
Figure 5
Figure 5
Two-dimensional scheme of the GA interactions with aldose reductase receptor.
Figure 6
Figure 6
Two-dimensional scheme of the GA interactions with glycogen phosphorylase receptor.
Figure 7
Figure 7
Results of α-amylase inhibitory activity. Values are expressed as mean ± standard deviation (SD, n = 3).
Figure 8
Figure 8
Results of α-glucosidase inhibitory activity. Values are expressed as mean ± SD (n = 3).
See this image and copyright information in PMC

References

    1. Es-Safi I., Mechchate H., Amaghnouje A., Jawhari F.Z., Bari A., Cerruti P., Avella M., Andriy A., Andriy D. Medicinal Plants Used to Treat Acute Digestive System Problems in the Region of Fez-Meknes in Morocco: An Ethnopharmacological Survey. Ethnobot. Res. Appl. 2020;20 doi: 10.32859/era.20.25.1-14. - DOI
    1. Parveen A., Parveen B., Parveen R., Ahmad S. Challenges and Guidelines for Clinical Trial of Herbal Drugs. J. Pharm. Bioallied Sci. 2015;7:329. doi: 10.4103/0975-7406.168035. - DOI - PMC - PubMed
    1. Atanasov A.G., Waltenberger B., Pferschy-Wenzig E.-M., Linder T., Wawrosch C., Uhrin P., Temml V., Wang L., Schwaiger S., Heiss E.H., et al. Discovery and Resupply of Pharmacologically Active Plant-Derived Natural Products: A Review. Biotechnol. Adv. 2015;33:1582–1614. doi: 10.1016/j.biotechadv.2015.08.001. - DOI - PMC - PubMed
    1. Süntar I. Importance of Ethnopharmacological Studies in Drug Discovery: Role of Medicinal Plants. Phytochem. Rev. 2020;19:1199–1209. doi: 10.1007/s11101-019-09629-9. - DOI
    1. Thomford N., Senthebane D., Rowe A., Munro D., Seele P., Maroyi A., Dzobo K. Natural Products for Drug Discovery in the 21st Century: Innovations for Novel Drug Discovery. IJMS. 2018;19:1578. doi: 10.3390/ijms19061578. - DOI - PMC - PubMed

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