Upconversion nanoparticles-based FRET system for sensitive detection of Staphylococcus aureus

Abstract

Staphylococcus aureus (S. aureus) is a pathogenic bacterium that seriously endangers food safety. Herein, a rapid, sensitive and specific aptasensor based on upconversion fluorescence resonance energy transfer (FRET) was developed for S. aureus detection in food. Aptamer-functionalized gold nanoparticles (AuNPs-aptamers) were bonded to cDNA-modified upconversion nanoparticles (UCNPs-cDNA) by complementary pairing, resulting in fluorescence quenching. After adding S. aureus into the system, the aptamers preferentially combined with S. aureus, dissociated UCNPs-cDNA from AuNPs-aptamers, and the fluorescence was recovered. Under optimized conditions, there was a significant linear correlation between fluorescence intensity and S. aureus concentration over the range 47-4.7 × 10⁷ CFU/mL (R² = 0.9904) with a detection limit of 10.7 CFU/mL. Furthermore, the precision and accuracy of the developed biosensor were validated using standard plate count method, yielding no significant differences. The proposed method has potential application for rapid and sensitive quantification of S. aureus in food.

Keywords: Aptamer; Fluorescence resonance energy transfer; Gold nanoparticles; Staphylococcus aureus; Upconversion nanoparticles.